FastQCFastQC Report
Fri 23 Jun 2023
ERR2382630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3073987
Total Bases412.1 Mbp
Sequences flagged as poor quality0
Sequence length35-135
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA219935871.54740732475446Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2160307.027680988891626Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA475111.5455823333019951Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA406651.3228748202253295RNA PCR Primer, Index 22 (96% over 27bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA306310.996458345464701Illumina PCR Primer Index 6 (100% over 28bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA222200.7228397517621252Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA201940.6569318608048765RNA PCR Primer, Index 40 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA194500.6327287656063607Illumina PCR Primer Index 6 (96% over 29bp)
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGT179970.5854611616770012No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA157790.5133073106685226Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC126900.41281892213597515Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC96200.312948623400164No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA85090.2768066358120577Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA73790.2400465584272152Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA65000.21145177256767841Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAACAAA62710.20400216396490942Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA59570.19378741679779388RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGCACCATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA58540.19043671947864452RNA PCR Primer, Index 32 (96% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA56120.18256420733074016Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA53230.17316273621196185Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAACAAAA51790.16847826617353945Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA47790.15546584940014385Illumina PCR Primer Index 6 (96% over 28bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA42570.1384846455108626Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAACAAAAA40350.13126275420162803Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAACAAAAAA39470.12840002251148103Illumina PCR Primer Index 6 (96% over 29bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA39210.12755421542121032Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGACAAAAAAA37000.12036485515390925Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA36780.11964917223137247Illumina PCR Primer Index 9 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA34240.11138628758026628Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCGGCTTGAAAAAAAAA31200.10149685083248564RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTGGAAAAAAAAA31180.10143178874861866Illumina PCR Primer Index 6 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)