FastQCFastQC Report
Fri 23 Jun 2023
ERR2382631_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382631_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761098
Total Bases37.3 Mbp
Sequences flagged as poor quality0
Sequence length39-50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA44755958.804385243424626Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA470816.1859313780879726Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA173412.278418810718199Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA84551.1108950489950045Illumina PCR Primer Index 6 (100% over 28bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA64120.8424670673159042Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA22840.3000927607220095Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA21070.27683688565730036RNA PCR Primer, Index 32 (96% over 28bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA20280.26645714480920984Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA15890.2087773190837448Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA15570.20457286709464484Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA13290.17461614667230763Illumina PCR Primer Index 9 (96% over 29bp)
ACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA12370.16252834720364526Illumina PCR Primer Index 6 (100% over 28bp)
ATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAA12220.16055751033375465Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCTCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA10550.13861552651563924Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA9800.12876134216618623Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA9650.12679050529629562Illumina Single End Adapter 1 (96% over 27bp)
CTGTAGGCACCACAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA9520.12508244667572377Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA9470.12442550105242689Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA9450.12416272280310814Illumina PCR Primer Index 6 (96% over 29bp)
AACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA8120.10668796922341144Illumina PCR Primer Index 6 (100% over 28bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA8120.10668796922341144Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA7800.10248351723431148RNA PCR Primer, Index 44 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)