FastQCFastQC Report
Fri 23 Jun 2023
ERR2382632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3336297
Total Bases164.1 Mbp
Sequences flagged as poor quality0
Sequence length37-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA160371048.068562241311255Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2839968.512311703664272Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1166853.4974404257174947Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA538551.6142148016198798Illumina PCR Primer Index 6 (100% over 28bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA437901.3125330268857958Illumina PCR Primer Index 6 (100% over 28bp)
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTATG354571.0627650955535433No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT252890.7579960656979879No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTATGC220320.660372862487962No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT157360.4716606465191798No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT153340.4596113595402328No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT147050.4407581219537709No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA141000.42262424478396254Illumina PCR Primer Index 6 (96% over 29bp)
TCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATATCTCGTATGCC131050.392800760843534No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA129880.38929387881234795Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA127130.381051207371526Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA124250.3724188823716833Illumina PCR Primer Index 6 (96% over 29bp)
CACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATGC116700.3497890025977903No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA95240.2854661920086851Illumina PCR Primer Index 6 (96% over 29bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT91140.2731771182241869No Hit
CTGTAGGCACCATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA75330.22578925077713408RNA PCR Primer, Index 32 (96% over 28bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA74650.223751062929949Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA74320.22276194235705035Illumina PCR Primer Index 6 (96% over 29bp)
AGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG73750.22105346136749815Illumina PCR Primer Index 6 (96% over 29bp)
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACC70110.21014316171491929No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA67690.20288961084699592No Hit
ATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC66890.20049174279148407Illumina PCR Primer Index 6 (96% over 26bp)
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT65020.19488672621172515No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATGCC62700.18793290885074082No Hit
TTTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATATCTCGTATG55680.16689161666362437No Hit
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA55370.16596244279211353Illumina PCR Primer Index 6 (96% over 29bp)
TCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATATCTCGTATGC53540.16047731961513018No Hit
AACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA52600.15765982464990377Illumina PCR Primer Index 6 (100% over 28bp)
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC48850.146419818139692No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATG44130.1322723966121721No Hit
CTGTAGGCACCATCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA40580.12163185711583831Illumina PCR Primer Index 9 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA39630.11878438879991798Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA39610.1187244420985302Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA37420.11216027829656651Illumina PCR Primer Index 6 (96% over 28bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC36660.10988230364383028No Hit
CTGTAGGCACCATCAATAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA34760.10418736701198965Illumina Single End Adapter 1 (96% over 27bp)
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT34220.10256880607451915No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)