FastQCFastQC Report
Fri 23 Jun 2023
ERR2382633_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382633_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748705
Total Bases100.3 Mbp
Sequences flagged as poor quality0
Sequence length45-135
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA31843442.53130405166254Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA10646714.220153464982872Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA557707.448861701204079Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA339734.5375682010938885Illumina PCR Primer Index 6 (100% over 28bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA323174.3163862936670645Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA136401.8218123292885715Illumina PCR Primer Index 6 (100% over 28bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA80211.0713164731102371Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC77601.0364562811788354Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA69360.9263995832804643Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA68710.917717926286054Illumina PCR Primer Index 6 (96% over 29bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA35140.4693437335131994Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA27990.3738455065746856Illumina PCR Primer Index 6 (100% over 28bp)
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC27260.364095337950194TruSeq Adapter, Index 6 (96% over 26bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC22480.3002517680528379Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA20890.27901509940497254Illumina PCR Primer Index 6 (96% over 28bp)
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC18450.24642549468749372No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA18000.24041511676828656Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA16130.2154386574151368Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT15330.20475354111432406No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA14810.1978082155187958Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA14480.19340060504471054TruSeq Adapter, Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA13620.18191410502133684Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA13100.17496877942580857Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC12170.16254733172611377Illumina PCR Primer Index 6 (96% over 29bp)
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC11460.15306429100914246No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT10930.145985401459854Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA10670.14251273866208988Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA10440.1394407677256062RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9670.12915634328607395Illumina PCR Primer Index 6 (96% over 29bp)
GTCGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8940.11940617466158233Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA8770.11713558744765962Illumina PCR Primer Index 6 (96% over 29bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA8720.11646776767885883No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG8720.11646776767885883No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC8520.11379648860365564No Hit
GTAGGCACCCTCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8350.11152590138973294Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7990.10671759905436722RNA PCR Primer, Index 32 (96% over 28bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7690.10271068044156242Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA7620.10177573276524132RNA PCR Primer, Index 37 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)