FastQCFastQC Report
Fri 23 Jun 2023
ERR2382634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4886086
Total Bases655.2 Mbp
Sequences flagged as poor quality0
Sequence length45-135
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA272053255.67916733352626Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA51486310.537329879171182Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2926435.989313327681912Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC1262042.5829262931516146No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA926221.8956277069212453Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC487110.9969329234074062Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA475920.9740311570447184Illumina PCR Primer Index 6 (100% over 28bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT437040.894458263730929No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA416390.8521953972975507Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA188430.38564609791968457Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC142880.29242219641651823Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA132970.27214011378432557Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA124410.25462097883663937Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA123270.2522878230141672Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA105800.2165332333487376Illumina PCR Primer Index 6 (96% over 28bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA105090.2150801275294786Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA103030.210864074025713Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA101350.20742573912943818RNA PCR Primer, Index 37 (96% over 27bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA93450.19125737860528857Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC91930.1881465041753256Illumina PCR Primer Index 6 (100% over 28bp)
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT91740.18775764487158023No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAACAAA87000.17805662855709048Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA84400.17273539597952225Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA78570.16080355523828274Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA77980.15959604476875763RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGCACCATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA71310.1459450365793807RNA PCR Primer, Index 32 (96% over 28bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA65740.13454531909589804Illumina PCR Primer Index 6 (96% over 29bp)
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG63820.13061579350015534No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA63330.12961294582207517Illumina PCR Primer Index 6 (100% over 28bp)
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC63200.12934688419319676No Hit
ATTACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA62710.1283440365151166TruSeq Adapter, Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAACAAAA61760.12639973999638976Illumina PCR Primer Index 6 (96% over 29bp)
AGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGC58620.1199733283450189No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAACAAAAA58210.11913421090009467Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT57420.11751737484767971Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA55670.11393577599739342Illumina PCR Primer Index 9 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA51830.10607672480590805Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)