FastQCFastQC Report
Fri 23 Jun 2023
ERR2382635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1513718
Total Bases202.9 Mbp
Sequences flagged as poor quality0
Sequence length45-135
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA57148737.7538616836161Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA22169214.64552842735569Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1023066.758590437584807Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA546043.6072769168365575Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA406022.682269749054976Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA307292.0300346563891027Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA306532.025013906156893Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA266551.7608960189414407Illumina PCR Primer Index 6 (100% over 28bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA209551.3843397515257136Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA167981.109717926324454Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA164271.085208737690904Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA162201.0715337995584382Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA104070.6875124692974517Illumina PCR Primer Index 6 (100% over 28bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT65710.4340967075769728No Hit
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA63790.4214127069903377Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA57550.38018970508377387TruSeq Adapter, Index 6 (96% over 29bp)
CAAGCTGTAGGCGCCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA41500.27415938767987164Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA37440.24733801143938303No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA35070.23168119821525543Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC34770.22969932312359367Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCCAATATCTCGTATGCCGTCTTCTGCTTGAAAA33670.2224324477875007Illumina PCR Primer Index 6 (96% over 30bp)
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA31690.20935207218253332Illumina PCR Primer Index 6 (96% over 29bp)
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG28050.1853053210703711No Hit
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA27750.1833234459787094Illumina PCR Primer Index 6 (96% over 29bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA24350.1608621949398765Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA22080.14586600674630282RNA PCR Primer, Index 37 (96% over 27bp)
ACGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA21760.1437520066485303Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA21200.14005250647742842RNA PCR Primer, Index 32 (96% over 28bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA21150.13972219396215146No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG21120.1395240064529853No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA21000.13873125641632061Illumina PCR Primer Index 6 (96% over 29bp)
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA20160.1331820061596678Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC18640.12314050569524838No Hit
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA18560.12261200567080525Illumina PCR Primer Index 6 (100% over 28bp)
ACAGATTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG17970.11871431799053722No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA17790.11752519293554016Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC17500.11560938034693383Illumina PCR Primer Index 6 (96% over 29bp)
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG17430.1151469428255461No Hit
ACAGACTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG17310.11435419278888143No Hit
CAAGCTGTAGGCACCATCGATATCTCGTATGCCGTCTTCTGCTTGAAAAA17100.1129668802247182RNA PCR Primer, Index 31 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC16780.11085288012694572Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCAGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA16530.10920131755056094Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA16530.10920131755056094Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAACAAA16040.1059642549008468Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA15790.10431269232446203Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)