FastQCFastQC Report
Fri 23 Jun 2023
ERR2382638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases540 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA63171815.79295No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC1754244.3856No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT1069922.6748000000000003No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA826002.0650000000000004Illumina PCR Primer Index 6 (96% over 29bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT804852.012125No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA534081.3352No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA500911.252275Illumina PCR Primer Index 6 (96% over 29bp)
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC467791.169475No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA429051.072625Illumina PCR Primer Index 6 (100% over 28bp)
TCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT351960.8798999999999999No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC347660.86915No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA324410.8110249999999999Illumina PCR Primer Index 6 (96% over 29bp)
CTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT311820.7795500000000001No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAT257490.643725No Hit
CCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC235860.58965No Hit
GTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT234790.586975No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC230290.575725No Hit
CTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA225070.562675No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG221400.5535No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAT213860.53465No Hit
GCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT201490.503725No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATATC201040.5026No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT191490.47872499999999996No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA190300.47574999999999995Illumina PCR Primer Index 6 (96% over 29bp)
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT159630.399075No Hit
GTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA154020.38505No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTC143840.3596No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT143090.357725No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTC122370.305925No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT121610.304025No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA117910.294775No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA98560.2464No Hit
TTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT96320.2408No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA89120.2228Illumina PCR Primer Index 6 (100% over 28bp)
GCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC84620.21155000000000002No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATATCT81320.2033No Hit
TAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC78500.19624999999999998No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCACTGTAGGCACCATCAATATC74150.18537499999999998No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCTGTAGGCACCATCAATATCT73370.183425No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC71450.178625No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATGCTGTAGGCACCATCAATA71290.178225No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT66100.16525No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATATCT61750.15437499999999998No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCG60660.15165No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC59300.14825000000000002No Hit
GGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG52410.131025No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT51470.12867499999999998No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA50070.125175No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTACTGTAGGCACCATCAATATCTC47770.119425No Hit
CCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAT47720.1193No Hit
GAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT46200.1155No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCTGTAGGCACCATCAATATC45440.1136No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTAT45190.11297499999999999No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAT44080.11019999999999999No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGCACCATCAATAT43920.10980000000000001No Hit
GGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCGT43540.10885No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT43080.1077No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATATCTCG42860.10715No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA42600.1065Illumina PCR Primer Index 6 (96% over 29bp)
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT40120.1003No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)