FastQCFastQC Report
Fri 23 Jun 2023
ERR2382644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752655
Total Bases236.6 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA27034715.424998074350057No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC751974.290462184514351No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT462932.6413070455965375No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT347471.9825350682250644No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA343011.9570879608365592Illumina PCR Primer Index 6 (96% over 29bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA234541.3381983333856349No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA223771.276748704108909Illumina PCR Primer Index 6 (96% over 29bp)
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC202561.1557323032770284No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA197131.1247507353129964Illumina PCR Primer Index 6 (100% over 28bp)
TCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT154100.8792374996790583No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC149150.8509946338554935No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA139430.795535915511039Illumina PCR Primer Index 6 (96% over 29bp)
CTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT135820.7749385931629442No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAT116830.6665886897307228No Hit
CCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC103880.5927007882327098No Hit
GTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT102160.5828871055627034No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC99690.5687942007982175No Hit
CTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA97340.5553859715688484No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAT92300.526629599093946No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG91840.5240050095426653No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT88270.5036359123729428No Hit
GCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT87190.49747383255689226No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATATC86500.4935369482299711No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA82900.4729966821764694Illumina PCR Primer Index 6 (96% over 29bp)
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT73770.42090428521300544No Hit
GTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA67940.38764046546525127No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTC62110.35437664571749716No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT60070.3427371616205129No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTC55200.31495074615369256No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT53190.30348243094048744No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA52120.29737740741903No Hit
TTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT43990.250990639914872No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA41940.23929409952329464Illumina PCR Primer Index 6 (100% over 28bp)
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA39760.2268558273020075No Hit
GCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC38230.2181262142292693No Hit
TAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC34960.19946880589733862No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATATCT34610.1974718355865815No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCACTGTAGGCACCATCAATATC30670.17499165551691576No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT30290.17282351632237947No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATGCTGTAGGCACCATCAATA29990.17111182748458767No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC29900.17059832083325013No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCTGTAGGCACCATCAATATCT29130.16620498614958448No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATATCT26260.1498298296013762No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTCG25360.14469476308800078No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC24870.14189900465294084No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA22470.12820549395060635No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT22430.12797726877223414No Hit
GGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG22160.12643674881822148No Hit
CCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAT21220.12107345712647384No Hit
GAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT20720.11822064239682083No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGCACCATCAATAT19480.11114566186728135No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTAT19220.1096621982078618No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTACTGTAGGCACCATCAATATCTC18960.10817873454844222No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAT18880.10772228419169774No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA18780.10715172124576713Illumina PCR Primer Index 6 (96% over 29bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT18710.10675232718361573No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT18630.10629587682687122No Hit
GGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCGT18380.10486946946204473No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCTGTAGGCACCATCAATATC18120.10338600580262515No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)