FastQCFastQC Report
Fri 23 Jun 2023
ERR2382653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559584
Total Bases83.7 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA15937928.48169354377538Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA391867.002702007205352Illumina PCR Primer Index 6 (100% over 28bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA271914.859145365128381Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA201143.5944558815119807Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA110891.9816506547721164Illumina PCR Primer Index 6 (96% over 29bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA110881.9814719505918683Illumina PCR Primer Index 6 (100% over 28bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA81381.4542946188597243Illumina PCR Primer Index 6 (96% over 29bp)
GACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA63281.1308400526105107Illumina PCR Primer Index 6 (96% over 29bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC57821.033267570195002No Hit
TGGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTG52280.9342654543375079No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA45440.8120317950477497Illumina PCR Primer Index 6 (100% over 28bp)
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA42660.7623520329387545No Hit
GGGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTG42320.7562760908103162No Hit
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA39170.6999842740321381Illumina PCR Primer Index 6 (96% over 29bp)
ACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT33680.6018756790758849No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA31570.564169097043518No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC27600.4932235374849888No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA25200.45033453422542463Illumina PCR Primer Index 6 (96% over 29bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT24730.44193543775376No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA22760.4067307142448676Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG18970.33900182993080574No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG18650.33328329616286384No Hit
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG18540.3313175501801338No Hit
CGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG17500.31273231543432267No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG17400.3109452736318408No Hit
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG17270.3086221192886144No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA15120.2702007205352548Illumina PCR Primer Index 6 (96% over 29bp)
ATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCT14740.26340996168582376No Hit
GGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTGT14460.2584062446388746No Hit
AGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGC14020.25054326070795446No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG13110.2342811803053697No Hit
ACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA12700.22695430891519416Illumina PCR Primer Index 6 (96% over 29bp)
TAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGCGAAACTCAGGT12600.22516726711271232No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC12470.22284411276948587No Hit
TCGGACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA12320.22016355006576313Illumina PCR Primer Index 6 (96% over 29bp)
AGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGTCGCGG11040.19728941499399552No Hit
AAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTAC10890.19460885229027278No Hit
CTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA10780.19264310630754275Illumina PCR Primer Index 6 (100% over 28bp)
AAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGCGAAACTCAGGT10660.19049865614456454No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTAA10610.18960513524332362No Hit
ATTTAGAGGAACTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGG10560.1887116143420827No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA10410.18603105163835992Illumina PCR Primer Index 6 (96% over 29bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT10180.18192085549265166No Hit
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG10160.18156344713215533No Hit
TGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG10140.18120603877165894No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA9670.17280694229999427No Hit
AGGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTG9440.16869674615428604No Hit
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCA9220.16476525418882598No Hit
AGTGGGTGACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCA9040.16154857894435867No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT9010.1610124664036141No Hit
AGAAAGCACCGTTTCCCGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCC8730.15600874935666495No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC8530.15243466575170125No Hit
GCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG8430.1506476239492194No Hit
GCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA8340.14903928632698576Illumina PCR Primer Index 6 (100% over 28bp)
ACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTC8160.14582261108251846No Hit
TCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA7840.14010407731457653Illumina PCR Primer Index 6 (100% over 28bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCCTGTAGGCACC7480.1336707268256419No Hit
CTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG7420.1325985017441528No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC7380.13188368502316006No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC7240.12938182649968547No Hit
CGGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTG6980.1247355178132327No Hit
ATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATATCTCG6880.12294847601075085No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATATCTCGTA6870.12276977183050267No Hit
GAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA6850.12241236347000628No Hit
TAGTGTAGTGGGTGACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAG6820.12187625092926174No Hit
AGAAAGCACCGTTTCCCGTCCGATCAACTGTAGGCACCATCAATATCTCG6690.11955309658603534No Hit
TGCGACTGTTAATCGCAAGATCGTGAGTTCAACCCTCACTGGGGTCGCCA6590.11776605478355351No Hit
CTGAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6290.11240492937610796Illumina PCR Primer Index 6 (100% over 28bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA6170.11026047921312974No Hit
AAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTGTAGGCACCAT5830.10418453708469148No Hit
CTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG5800.10364842454394693No Hit
TAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGAC5740.10257619946245783No Hit
GGACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA5720.10221879110196144Illumina PCR Primer Index 6 (96% over 29bp)
TGCGACTGTTAATCGCAAGATCGTGAGTTCACCCC5610.10025304511923144No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)