FastQCFastQC Report
Thu 22 Jun 2023
ERR2382660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Total Bases540 Mbp
Sequences flagged as poor quality0
Sequence length135
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA3289368.2234No Hit
AAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT1545503.86375No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1171632.929075No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA1094402.7359999999999998No Hit
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA997572.493925Illumina PCR Primer Index 6 (96% over 29bp)
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT645631.614075No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA567251.4181249999999999Illumina PCR Primer Index 6 (96% over 29bp)
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC561181.4029500000000001No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA498611.246525Illumina PCR Primer Index 6 (96% over 29bp)
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAA423211.058025No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA418601.0465No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT414431.036075No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATATC408951.022375No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC381490.953725No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT347330.868325No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAA342630.8565750000000001No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA335750.839375Illumina PCR Primer Index 6 (100% over 28bp)
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT277550.6938749999999999No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA274140.68535Illumina PCR Primer Index 6 (96% over 29bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT270470.6761750000000001No Hit
TGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAA239630.599075No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT233350.5833750000000001No Hit
TCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT181850.454625No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT181630.45407499999999995No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCA178640.44660000000000005No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA174680.43670000000000003No Hit
CTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT174530.436325No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCA166080.4152No Hit
CTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA161260.40315No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCA160810.402025No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT157960.39490000000000003No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAA137660.34415No Hit
GTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT134030.335075No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCA131820.32955No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTCG121570.303925No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC119710.299275No Hit
GTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA114960.2874No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTGTAGGCACCATCAAT109840.2746No Hit
GCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT100500.25125No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT99570.248925No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAA97630.24407500000000001No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA93100.23275No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAT93000.23249999999999998No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTGTAGGCACCATCAAT87690.21922500000000003No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAT87080.2177No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCTGTAGGCACCATCAA85870.21467499999999998No Hit
CCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC84350.210875No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTC75660.18914999999999998No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA75120.1878No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT72170.180425No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT71520.17880000000000001No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA71460.17864999999999998Illumina PCR Primer Index 6 (100% over 28bp)
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA69710.17427499999999999No Hit
TTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT68750.171875No Hit
CTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA66850.167125No Hit
TAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC65720.1643No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCGT64900.16225No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCTC63160.1579No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGTAGGCACCATCAATAT63080.1577No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATATCTC62630.15657500000000002No Hit
CAAGCAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA60580.15145Illumina PCR Primer Index 6 (96% over 29bp)
GTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAAT58960.1474No Hit
CGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAA56270.140675No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA56080.1402No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC53380.13344999999999999No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATATC52650.131625No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATGCTGTAGGCACCATCAATA51770.129425No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCTGTAGGCACCATCAATATC51540.12885No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATATCT51380.12845No Hit
TGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCA50090.125225No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT48130.12032499999999999No Hit
AGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCCTGTAGGCACCATCAA47600.11900000000000001No Hit
GGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATATC45820.11455No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCTGTAGGCACCATCAATATCT45380.11345000000000001No Hit
GGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATC44730.11182500000000001No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCACTGTAGGCACCATCAATATC42630.106575No Hit
TAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT41930.10482499999999999No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA41850.10462500000000001Illumina PCR Primer Index 6 (100% over 28bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA40560.10139999999999999Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)