FastQCFastQC Report
Fri 23 Jun 2023
ERR2382684_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382684_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471887
Total Bases331.2 Mbp
Sequences flagged as poor quality0
Sequence length35-135
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA61367524.8261753065573Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1908757.7218335627801755Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1317205.328722550828577Illumina PCR Primer Index 6 (96% over 29bp)
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATC1031544.173087200183503No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC589772.3859100355315594No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA495292.0036919163376Illumina PCR Primer Index 6 (100% over 28bp)
TTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCC463571.875368898335563No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAA332401.34472166405665No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT310241.2550735531195398No Hit
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA304101.2302342299627775Illumina PCR Primer Index 6 (96% over 29bp)
AAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAG268771.0873069845021233No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA251991.0194236225199615Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA243240.9840255642753896Illumina PCR Primer Index 6 (96% over 29bp)
TATCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC232910.9422356280849408No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA180900.7318295698792057Illumina PCR Primer Index 6 (96% over 29bp)
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGT160370.648775611506513No Hit
GGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATC147710.597559678092081No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA130230.5268444714503535Illumina PCR Primer Index 6 (96% over 29bp)
TGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAA121640.492093691985111No Hit
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG112020.4531760553779359No Hit
TTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGA107170.43355541737951614No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT95240.38529269339577416No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA92390.37376304013897077No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA91850.37157847425873436Illumina PCR Primer Index 6 (96% over 29bp)
TGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG84000.33982135914788986No Hit
CGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG81430.32942444375491275No Hit
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC75960.3072956004865918TruSeq Adapter, Index 6 (96% over 26bp)
TATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAG74450.3011869070066714No Hit
AGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATC74030.29948780021093196No Hit
TTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG72270.29236773363830953No Hit
CGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATC66700.2698343411329078No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA66210.26785204987121175Illumina PCR Primer Index 6 (100% over 28bp)
TATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGA65050.2631592787210742No Hit
GTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCC61060.24701776416154947No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC60600.2451568376709777No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCT57110.231038069296857No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA50620.20478282381031174Illumina PCR Primer Index 6 (96% over 29bp)
ATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAG48780.1973391178480246No Hit
TCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGC47490.192120432689682No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTAGAGGAACTAAAAGTCGTA46380.1876299361580849No Hit
ATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTA43200.1747652704189148No Hit
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG40490.16380198609402452No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG39010.15781465738522837No Hit
CGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTG37710.15255551730317768No Hit
TTGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTA37450.15150368928676755No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT35940.14539499580684714No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC35160.14223951175761673No Hit
TTAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACCTTTATAGTTTCT32770.13257078499138514No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA31550.12763528429899912Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA30480.12330660746223432Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA30350.12278069345402925Illumina PCR Primer Index 6 (100% over 28bp)
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGG29920.12104113173458171No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTATTGCTCGAAT29570.11962520940479884No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG28470.11517516779690981No Hit
TCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA28260.11432561439904008Illumina PCR Primer Index 6 (100% over 28bp)
CCTGCGGAAGGATCATTCTGTAGGCACCATCAATATCTCGTATGCCGTCT27960.11311196668779763Illumina PCR Primer Index 6 (95% over 21bp)
CCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTATTGCTCGAA27390.11080603603643693No Hit
TCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCG26580.10752918721608229No Hit
TCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAG26360.10663917889450449No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG26070.10546598610697011No Hit
TTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTG25320.10243186682886395No Hit
AAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCA25300.10235095698144779No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA24880.10065185018570832Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)