FastQCFastQC Report
Fri 23 Jun 2023
ERR2382693_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382693_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692343
Total Bases92.8 Mbp
Sequences flagged as poor quality0
Sequence length121-135
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACCA277244.004373554726487No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA221703.2021700226621777Illumina PCR Primer Index 6 (96% over 29bp)
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC164452.375267750233627TruSeq Adapter, Index 6 (96% over 26bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG143072.0664612771415323No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCA126981.8340620183926175No Hit
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG108321.5645424305582638No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCCTG106181.5336328958334235No Hit
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCC79851.1533300690553672No Hit
GTCCTGGCGGGGAAGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT75121.0850113310887812Illumina PCR Primer Index 6 (95% over 21bp)
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC70141.0130816661683588No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC68480.9891051111948845No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCC66220.9564623315322031No Hit
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG64320.9290192866830459No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC63560.918042068743383No Hit
CCTGATTCAAGCTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGC61710.8913212092849931No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCC59410.8581006813096977No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC59190.8549230655903215No Hit
ATCCGGGTAGGACCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT58010.8378794903682134Illumina PCR Primer Index 6 (95% over 21bp)
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC56090.8101475713627494No Hit
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC54690.7899263804212652No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC54680.7897819433431118No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC50890.735040290722951No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG47530.6865094324633888No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG46930.6778432077741813No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA43740.6317677798432281Illumina PCR Primer Index 6 (100% over 28bp)
TCCTGGGCGAAATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT43440.6274346674986242Illumina PCR Primer Index 6 (95% over 21bp)
CCCTCACTGGGGTCGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT41260.5959473844611702Illumina PCR Primer Index 6 (95% over 21bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA39010.563449041876642Illumina PCR Primer Index 6 (96% over 29bp)
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG38410.5547828171874346No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCACT38270.5527606980932861No Hit
TCCCGCTAGAGACCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT37000.5344171891677969Illumina PCR Primer Index 6 (95% over 21bp)
GTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTC33890.4894972578620712No Hit
GTCCTGGCGGGGAAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC32640.471442623092889No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC32330.46696507367013174No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC32170.46465408041967643No Hit
GTCCTGGCGGGGAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT30840.4454439490252664RNA PCR Primer, Index 6 (95% over 22bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCCT30490.44038865128989535No Hit
GCGGCGCTACCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT28090.40572375253306525Illumina PCR Primer Index 6 (96% over 27bp)
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC27900.40297944804814956No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCA27810.40167951434476845No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA27710.40023514356323386Illumina PCR Primer Index 6 (100% over 28bp)
TCCTGAGTGCGAGCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT27620.39893520985985265Illumina PCR Primer Index 6 (95% over 21bp)
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCAC27100.39142448179587286No Hit
TCCACAGAATTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC26660.38506925035712064No Hit
GGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCTT26470.38232494587220495No Hit
CGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCACTGTAGGCACCA24090.3479489212716818No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCA24080.3478044841935284No Hit
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA21760.31429508206192597Illumina PCR Primer Index 6 (96% over 29bp)
CTCCCTTGGCTTGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCC21060.30418448659118386No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCGG20480.29580713605828324No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA19110.2760192563512594Illumina PCR Primer Index 6 (96% over 29bp)
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC19010.27457488556972487No Hit
CCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACCA18790.27139726985034873No Hit
TCCACAGAATTCGCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT18630.2690862765998934Illumina PCR Primer Index 6 (95% over 21bp)
AGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGTCGCGG18040.2605644889888393No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC17720.25594250248792866No Hit
TGGCAACTTGGCCGAGTGGTTAAGGCAAAAGATTGGAAATCTTTTGGGCT17450.2520427013777853No Hit
CCCCCCATCGTGAGTGCCCTGTAGGCACCATCAATATCTCGTATGCCGTC17300.2498761452054834No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCCT17140.24756515195502807No Hit
GCTCAATGGTAGAGCTTTCGACTCCAAATCGAAGGGTTGCAGGTTCATTT16680.24092104635996897No Hit
TATGACTCTTAATCATAAGGTTAGGGGTTCGAGCCCCCTACAGGGCTCCC16060.2319659475144545No Hit
CTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG15760.22763283516985078No Hit
CTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG15650.22604402731016277No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCTGTAGGCACCAT15510.2240219082160143No Hit
ACCTCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT15270.22055541834033132Illumina PCR Primer Index 6 (95% over 21bp)
TCTCCTTCCTAAGGAGAAGACTGCGGGTTCGAGTCCCGTACGGAACGCCA15260.22041098126217787No Hit
ACCTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA15070.21766667677726215Illumina PCR Primer Index 6 (96% over 29bp)
GTCTAGTGGTATGATTCTCGCTTTGGGTGCGAGAGGCCCTGGGTTCATTT14590.21073369702589612No Hit
TCCTGGGCGAAATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT14570.2104448228695892Illumina PCR Primer Index 6 (95% over 24bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA14520.20972263747882194Illumina PCR Primer Index 6 (96% over 29bp)
TGCGACTGTTAATCGCAAGATCGTGAGTTCAACCCTCACTGGGGTCGCCA14440.20856714085359424No Hit
TCCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACC14140.2042340285089905No Hit
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC14000.20221190941484204No Hit
ATCCGGGTAAGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC13990.2020674723366886No Hit
ATCCGGGTAAGACCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT13330.19253462517856032Illumina PCR Primer Index 6 (95% over 21bp)
AAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTCCGGGCTTGC13320.19239018810040687No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCATCGTGAGTGCCTGT13290.1919568768659465No Hit
CAAGACTGTAAATCTTGAGATCGGGCGTTCGACTCGCCCCCGGGAGACCA12950.1870460162087289No Hit
CCTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACC12710.18357952633304592No Hit
ATCCCTTAGCTCTCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT12480.18025747353551635Illumina PCR Primer Index 6 (95% over 21bp)
GCGGCGCTACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT12420.1793908510665956Illumina PCR Primer Index 6 (96% over 28bp)
TCTGACTTCTAATCAGAAGATTATGGGTTCCCCCCCATCGTGAGTGCCAC12340.17823535444136793No Hit
ATCCGGGTAGGACCTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT12100.1747688645656849Illumina PCR Primer Index 6 (95% over 22bp)
TCTCCTTCCTAAGGAGAAGACTGCGGGTTCGAGTCCCGTACGGAACGCCC12040.1739022420967642No Hit
ACAGATTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG11920.17216899715892267No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGGGCCCCCTATGAGGAGCCA11780.17014687806477424No Hit
ATCTGGAGGTCCTGTGTTCGATCCACAGAATTCGCACCACTGTAGGCACC11770.1700024409866208No Hit
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC11570.16711369942355161No Hit
TCCTGGGCGAAATCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC11380.16436939493863592No Hit
ACAGACTTGAAATCTGTTGGGCTCTGCCCGCGCTGGTTCAAATCCTGCTG11200.16176952753187365No Hit
GCCCCCTATGAGGAGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT11090.1601807196721856Illumina PCR Primer Index 6 (95% over 21bp)
TCTGACTTCTAATCAGAAGATTATGGGTCCCCCCCATCGTGAGTGCCCTG10820.15628091856204224No Hit
CGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT10740.15512542193681456TruSeq Adapter, Index 6 (96% over 27bp)
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAAGTCCTGGCGGGGAAGCCC10670.15411436238974033No Hit
ATCCTGCTGGTGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC10530.15209224329559193No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGTGCCAGATCGGGG10220.1476146938728347No Hit
AAAGATTGGAAATCTTTTGGGCTTTGCCCGCGCAGGTTCGAGTCCTGCAG10180.14703694556022087No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACTGTAGGCACCATC10140.14645919724760706No Hit
GCCCCCTATGAGGAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT10030.144870389387919TruSeq Adapter, Index 6 (95% over 22bp)
ATCCGGGTGGGACCTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT9790.14140389951223598Illumina PCR Primer Index 6 (95% over 21bp)
CTTCCCTTGCAAGGAAGAGGTCATCGGTTCGATTCCGGTTGCGTCCACCA9670.13967065457439448No Hit
GCCCCCTACAGGGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC9650.13938178041808758No Hit
CTCGCCCCCGGGAGACCTGTAGGCACCATCAATATCTCGTATGCCGTCTT9420.136059727620558Illumina PCR Primer Index 6 (95% over 22bp)
ACCCCGCGGGTATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC9390.13562641638609765No Hit
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC9340.13490423099533033No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGAGCCCCCTATGAGGAGCCA9270.13389317144825613No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGACCCCCATCGTGAGTGCCT9210.13302654897933539No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCCTGTAGGCACCA8720.12594913214981593No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8710.12580469507166248Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA8570.12378257597751403Illumina PCR Primer Index 6 (100% over 28bp)
TGCGACTGTTAATCGCAAGATCGTGAGTTCAACCCTCACTGGGGTCGCCC8350.12060496025813794No Hit
TCCCGCTAGAGACCACCTGTAGGCACCATCAATATCTCGTATGCCGTCTT8330.12031608610183102Illumina PCR Primer Index 6 (95% over 22bp)
TCCTGGGCGAAATCGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT8280.11959390071106374Illumina PCR Primer Index 6 (95% over 21bp)
TCTGGCTGCGAACCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGAAGCCC8030.11598297375722726No Hit
TGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCCCCACTGTAGGCACC7950.1148274771319996No Hit
TTCGGCTTTTAACCGAAATGTCAGGGGTTCGTGCCCCCTATGAGGAGCCA7860.11352754342861847No Hit
TCCTGAGTGCGAGCTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT7830.11309423219415811Illumina PCR Primer Index 6 (95% over 22bp)
ATCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCACCCTGTAGGCACCA7800.11266092095969771No Hit
ATGCCTTGCTAAGGCATTGGGTTTTACCTGCGCAGGTTCGAATCCTGTCT7650.11049436478739584No Hit
TCAGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGTCGC7650.11049436478739584No Hit
GTCTAGTCGGTTATGGCATCTGCTTGACACGCAGAACGTCCCCAGTTCGT7450.10760562322432667No Hit
AAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCG7400.10688343783355939No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA7380.10659456367725247Illumina PCR Primer Index 6 (96% over 29bp)
TCCTCGGTTGGAACACCACTGTAGGCACCATCAATATCTCGTATGCCGTC7310.10558350413017825No Hit
TCTGACTTCTAATCAGAAGATTATGGGTTCGCCCCCCATCGTGAGTGCCT7260.10486131873941097No Hit
CCACATTGGTAATGTGGAGATCATCGGTTCATCCGATTGGAAGCACCACT7200.10399469627049021No Hit
TTCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCG7190.10385025919233674No Hit
GTCTAGTCGGTTATGGCATCTGCTTGACACGCAGAACGTCCCCAGTTCGA7060.10197257717634178No Hit
TCAGATTTAGGCTCTGATATCTTCGGATGCAAGGGTTCGAATCCCTTAGC6970.10067264347296066No Hit
ATCGTGAGTGCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC6950.10038376931665374Illumina PCR Primer Index 6 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)