FastQCFastQC Report
Thu 22 Jun 2023
ERR2382700_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382700_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485174
Total Bases333.2 Mbp
Sequences flagged as poor quality0
Sequence length35-135
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA62300625.06890865589291Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1296425.216616623222358Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1177374.73757571904422Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA569542.291751000131178Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA527422.1222658856080097Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA474401.908920663100451Illumina PCR Primer Index 6 (100% over 28bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA372191.4976416138266375Illumina PCR Primer Index 6 (96% over 29bp)
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT280001.126681672993521No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA212220.853944230866732Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA205730.8278293592319894Illumina PCR Primer Index 6 (100% over 28bp)
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCGT203160.817488031019156No Hit
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA181300.7295263832633048Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA138900.558914587067143No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCGTA126900.5106282296531349No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATG114320.4600080316307832No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTA98810.3975979146731778No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA97420.3920047449393885Illumina PCR Primer Index 6 (96% over 29bp)
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCGT90990.36613130509171593No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTATGC83450.33579137718324753No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA80830.32524885581452245Illumina PCR Primer Index 6 (96% over 29bp)
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTC79220.3187704361948097No Hit
TTTCGGTGCCCGAGTTGTAATTTCTGTAGGCACCATCAATATCTCGTATG68590.27599677125223426No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA66060.26581639756411424Illumina PCR Primer Index 6 (96% over 29bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCT65390.26312040927516545No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT61540.24762853627150455No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATATCTCGTAT59410.2390577078305181No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATCTC56000.22533633459870414No Hit
TTATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCGT53730.21620216532122097No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAT51960.20907992760265479No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATATCTCGTAT50750.20421105323007566No Hit
TAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCGTATG50470.20308437155708214No Hit
GAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATATCTCGTA49330.19849716760275132No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA48530.19527807710848416Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA47480.19105302083475845Illumina PCR Primer Index 6 (100% over 28bp)
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATATCTCGTATGC38510.15495896866778744No Hit
CTGTAGGTACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA33600.1352018007592225Illumina PCR Primer Index 6 (100% over 28bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATATC30500.12272782509393709No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCGTATGC29040.11685298494189943No Hit
TTAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCGTAT28350.11407651939059399No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATATCTCGTAT27980.1125876900369954No Hit
TATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATATCTCG26990.10860406555033973No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATATC26900.10824191786973467No Hit
CCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATG26730.10755786113970289No Hit
GCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATATCTCGTATG26720.10751762250852455No Hit
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA26230.1055459295807859Illumina PCR Primer Index 6 (96% over 29bp)
ATAAATCATTTGTATACGACTTAGATCTGTAGGCACCATCAATATCTCGT25560.10284994129183711No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)