FastQCFastQC Report
Fri 23 Jun 2023
ERR2382706_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382706_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733842
Total Bases392.3 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA144933753.01465849160266Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA29388110.749743401410909Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA1877456.867441498082186Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC872093.189979523322855No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA756042.765485349921466Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA541601.981094737735392Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA437971.602031134206Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA264990.9692952262786217Illumina PCR Primer Index 6 (96% over 29bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA209330.7656989687041167Illumina PCR Primer Index 6 (96% over 29bp)
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT203420.7440810405283115No Hit
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA163530.5981691699812938Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA147000.5377048124946504Illumina PCR Primer Index 6 (96% over 29bp)
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA118340.4328706633375301Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA115510.42251893123304124Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA69140.2529041546658512Illumina PCR Primer Index 6 (100% over 28bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA66470.24313767950013207Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA62720.2294207199977175Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA57110.20890014858210532Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA56330.20604702100560313Illumina PCR Primer Index 6 (96% over 29bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA53660.19628054583988394Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACTATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA50280.1839169930083743Illumina PCR Primer Index 6 (100% over 28bp)
AAGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTG49830.18227095786808456Illumina PCR Primer Index 6 (96% over 29bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA48840.1786496805594471Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGTACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA47800.17484551045744415Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA46540.17023661206463286RNA PCR Primer, Index 32 (96% over 28bp)
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT44970.16449377835295528No Hit
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA41820.15297153237092706Illumina PCR Primer Index 6 (96% over 29bp)
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA40650.14869184100617372Illumina PCR Primer Index 6 (96% over 29bp)
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA39930.14605818478171012Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA37990.13896194439912768Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCTTCTTCTGCTTGAAAAAAA34570.1264520773329256RNA PCR Primer, Index 37 (96% over 27bp)
CCTGCGGAAGGATCATTACTTTAGGCACCATCAATATCTCGTATGCCGTC33580.12283080002428817No Hit
AGAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGA31020.11346668900397316Illumina PCR Primer Index 6 (96% over 29bp)
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCTGT30840.11280827494785726No Hit
AGATCGGAAGAGCGTCCTGTAGGCACCATCAATAT28750.10516335618517823Illumina Single End PCR Primer 1 (95% over 23bp)
CTGTAGGAACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA28660.10483414915712028Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA28530.10435862789436991RNA PCR Primer, Index 37 (96% over 27bp)
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC27880.10198102158061806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)