FastQCFastQC Report
Fri 23 Jun 2023
ERR2382719_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382719_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7718751
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length45-135
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA340291744.08636837747454Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA6081017.87823055828592Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA5891727.632996581959957Illumina PCR Primer Index 6 (100% over 28bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3788454.908112724455032Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA3043143.942529043882877Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2584533.348378513570395Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1522621.9726248456518418Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1470941.9056710081721768Illumina PCR Primer Index 6 (100% over 28bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA1044751.3535220918513888Illumina PCR Primer Index 6 (96% over 29bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA619940.8031610295499881Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA611110.7917213549186909Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA577660.7483853281444109Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA553330.7168646844547777Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA289870.37554003231870026TruSeq Adapter, Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC271990.35237566285011657No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT234690.30405178247102416No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA232070.3006574509269699Illumina PCR Primer Index 6 (96% over 29bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC213210.276223445995343No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC205280.26594976311582014Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA194260.2516728418885387RNA PCR Primer, Index 37 (96% over 27bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC188070.2436534097291129Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCGCCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA181820.23555624478623552Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA175960.22796434293579365Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA173610.22491980891727173Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA168370.2181311458291633Illumina PCR Primer Index 6 (96% over 29bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA148740.1926995701765739Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA143310.185664753274202Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA136350.17664775039381372RNA PCR Primer, Index 32 (96% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAACAAA122330.15848418999395109Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA109420.1417586860879435Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCGCCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA109020.14124046753159936Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA107520.13929714794530876RNA PCR Primer, Index 37 (96% over 27bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC104780.1357473508343513Illumina PCR Primer Index 6 (100% over 28bp)
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT94890.12293439702874209No Hit
CAAGCTGTAGGCACCATCCAATATCTCGTATGCCGTCTTCTGCTTGAAAA90430.11715626012550476Illumina PCR Primer Index 6 (96% over 30bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAA89760.1162882440436283RNA PCR Primer, Index 37 (96% over 27bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA89230.1156016044564723Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAACAAAA86650.11225909476805249Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA85720.11105423662455234Illumina PCR Primer Index 6 (96% over 29bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA80010.10365666673273954Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAACAAAAA79670.103216180959847Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)