FastQCFastQC Report
Fri 23 Jun 2023
ERR2382721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7834760
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length44-135
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA321573941.04451189315308Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA79072510.09252357442985Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA6167267.87166422455825Illumina PCR Primer Index 6 (100% over 28bp)
GTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3395034.333291638799402Illumina PCR Primer Index 6 (96% over 29bp)
TCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA2658893.393709571192991Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2195442.8021790073978017Illumina PCR Primer Index 6 (100% over 28bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1424951.8187538609989329Illumina PCR Primer Index 6 (96% over 29bp)
AAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA1344741.7163767620195132Illumina PCR Primer Index 6 (96% over 29bp)
CGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1327951.694946622487479Illumina PCR Primer Index 6 (100% over 28bp)
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA668220.8528914733827201Illumina PCR Primer Index 6 (100% over 28bp)
CAAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA644430.8225267908653232Illumina PCR Primer Index 6 (96% over 29bp)
TTGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC599300.7649245158754066No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA498690.6365096059100726Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA463810.591990054577294Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC410030.5233472371840363No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT393430.5021596066758905No Hit
CAAGCTGTAGACACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA283830.36227019079078365TruSeq Adapter, Index 6 (96% over 29bp)
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT236820.30226835282765524No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA213810.2728992336714845Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC180790.2307537180462452Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA174630.22289132021912605Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCGCCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA174140.22226590221015063Illumina PCR Primer Index 6 (96% over 29bp)
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC169310.21610106755025044Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAA166050.21194012324563866RNA PCR Primer, Index 37 (96% over 27bp)
CTGTAGGCACATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA151680.19359878285997273Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA151480.19334351020324808Illumina PCR Primer Index 6 (96% over 29bp)
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA150620.1922458377793321Illumina PCR Primer Index 6 (96% over 29bp)
AGCTGTAGGCGCCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA138190.17638064216389526Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA120850.1542485028258683Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCTGTAGGCACCATCCAATATCTCGTATGCCGTCTTCTGCTTGAAAA114830.14656479585845641Illumina PCR Primer Index 6 (96% over 30bp)
CTGTAGGCACCATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA112280.14331006948521716RNA PCR Primer, Index 32 (96% over 28bp)
AGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGC110280.14075734291797068No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC109620.13991494315077935Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAACAAA106340.1357284715804951Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA98750.12604087425779475Illumina PCR Primer Index 6 (96% over 29bp)
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA93230.11899534893219449Illumina PCR Primer Index 6 (100% over 28bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA87370.11151586009016229Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA85000.10849087910797524RNA PCR Primer, Index 37 (96% over 27bp)
ATGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGC82000.10466178925710552No Hit
CAGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA79400.1013432447196851Illumina PCR Primer Index 6 (96% over 29bp)
TGGATTTGAGTAAGAGCATAGCTGTAGGCACCATCAATATCTCGTATGCC79310.101228372024159No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)