FastQCFastQC Report
Fri 23 Jun 2023
ERR2382722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295446
Total Bases44.2 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2958710.014351184311177Illumina PCR Primer Index 6 (96% over 29bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG146244.94980470204369No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA114713.8826046045639475Illumina PCR Primer Index 6 (100% over 28bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC99273.360004873987125No Hit
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG56161.900854978574765No Hit
GCGTAATGGCAACGCGTCTGACTTCTAATCAGAAGATTATGGGTTCCCCC48091.6277086168030708No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC41251.3961942283869133No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA40101.3572700256561268Illumina PCR Primer Index 6 (96% over 29bp)
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG38831.3142841669882146No Hit
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG36911.2492976719942053No Hit
GCTCGCGTGGCGTAATGGCAACGCGTCTGACTTCTAATCAGAAGATTATG35541.2029271000453552No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC33961.1494486302065352No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC30521.033014493342269No Hit
GCGCAATCGGTAGCGCGTATGACTCTTAATCATAAGGTTAGGGGTTCGAG30291.0252296527961118No Hit
GTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTC29731.0062752584228591No Hit
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC23780.8048848182070497No Hit
GGCAACTTGGCCGAGTGGTTAAGGCGAAAGATTGGAAATCTTTTGGGCTT22630.765960615476263No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC22380.757498832273918No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC21390.7239901707926322No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG20220.6843890254056578No Hit
GCGCAGTTGGTAGCGCGCTTCCCTTGCAAGGAAGAGGTCATCGGTTCGAT20090.6799888981404385No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC19780.6694962869695309No Hit
GCCTTGTTGGCGCAATCGGTAGCGCGTATGACTCTTAATCATAAGGTTAG19660.6654346310324052No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG16840.5699857165099544No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC16660.563893232604266No Hit
CCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACCA16260.5503543794805142No Hit
GCGTAATGGCAACGCGTCTGACTTCTAATCAGAAGATTATGGGTTCGCCC16130.5459542522152948No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCGTTCGGCTTTTAACCGAAATGTCAG15540.5259844438577608No Hit
GCGTAATGGCAACGCGTCTGACTTCTAATCAGAAGATTATGGGTTCGACC15400.5212458452644476No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCCTGTAGGCACC14500.4907834257360059No Hit
TGGCAACTTGGCCGAGTGGTTAAGGCGAAAGATTGGAAATCTTTTGGGCT14350.485706355814599No Hit
TCCACAGAATTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC13940.4718290313627533No Hit
GCGTAGTCGGTAGCGCGCTCCCTTGGCTTGGGAGAGGTCTCCGGTTCGAT13450.45524393628615717No Hit
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC13270.44915145238046883No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCGG12680.4291816440229348No Hit
ATCTGGAGGTCCTGTGTTCGTTCCACAGAATTCGCACCACTGTAGGCACC11760.3980422818383055No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA10600.358779607779425Illumina PCR Primer Index 6 (96% over 29bp)
TCCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACC10350.3503178245770801No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC10180.3445638119994855No Hit
ATCTGGAGGTCCTGTGTTCGATCCACAGAATTCGCACCACTGTAGGCACC10090.34151757004664135No Hit
GCTCAATGGTAGAGCTTTCGACTCCAAATCGAAGGGTTGCAGGTTCATTT9800.33170190153192125No Hit
GGCCATCTTAGTATAGTGGTTAGTACACATCGTTGTGGCCGATGAAACCC9760.330348016219546No Hit
ATCCGGGTAAGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC8840.2992086540349167No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGTGCCAGATCGGGG8830.29887018270682286No Hit
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC8830.29887018270682286No Hit
ATCTGTTGGACGGTTGTCCGCGCGAGTTCGAACCTCGCATCCTTCACCAC8650.2927776988011345No Hit
TGGTTGTTTGGCCGAGCGGTCTAAGGCGCCTGATTCAAGCTCAGGTATCG8450.2860082722392586No Hit
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC8450.2860082722392586No Hit
GCGTAATGGCAACGCGTCTGACTTCTAATCCGAAGATTATGGGTTCCCCC7340.24843795482084713No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6750.2284681464633131Illumina PCR Primer Index 6 (100% over 28bp)
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC6530.22102177724524957TruSeq Adapter, Index 6 (96% over 26bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA6480.21932942060478056Illumina PCR Primer Index 6 (100% over 28bp)
GTTTAAGGCGCAAGACTGTAAATCTTGAGATCGGGCGTTCGACTCGCCCC6390.21628317865193641No Hit
CTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG6260.211883051386717No Hit
TGGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTG6070.2054520961529349No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC6050.2047751534967473No Hit
TGCTCGCGTGGCGTAATGGCAACGCGTCTGACTTCTAATCAGAAGATTAT5900.19969808357534033No Hit
GCGTAATGGCAACTCGTCTGACTTCTAATCAGAAGATTATGGGTTCCCCC5670.191913243029183No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCCCCGTGGAGACCGG5570.18852852974824502No Hit
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC5480.18548228779540085No Hit
GCTCGCGTGCGTAATGGCAACGCGTCTGACTTCTAATCAGAAGATTATGG5460.18480534513921326No Hit
GCTCGCGTTGCGTAATGGCAACGCGTCTGACTTCTAATCAGAAGATTATG5150.17431273396830554No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCTGTAGGCACCAT4990.1688971927188048No Hit
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA4970.16822025006261718Illumina PCR Primer Index 6 (100% over 28bp)
GCCCAGTTGGTTAAGGCACCGTGCTGATAACGCGGGGATCAGCGGTTCGT4880.165174008109773No Hit
CTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG4870.16483553678167923No Hit
TGGTAATGTGGAGATCATCGGTTCATCCGATTGGAAGCACCACTGTAGGC4840.16382012279739783No Hit
GTCTAGTGGTATGATTCTCGCTTTGGGTGCGAGAGGCCCTGGGTTCATTT4700.15908152420408467No Hit
TCCTCGGTTGGAACACCACTGTAGGCACCATCAATATCTCGTATGCCGTC4640.1570506962355219No Hit
GTCCTGGCGGGGAAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC4370.14791197037698936No Hit
GGGCGGTTAGTGTAGTGGTTATCATCCCACCCTTCCAAGGTGGGGACACG4240.14351184311177No Hit
ACCCCACCTCGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC4220.1428349004555824No Hit
GTCTAGTCGGTTATGGCATCTGCTTGACACGCAGAACGTCCCCAGTTCGA4050.13708088787798786No Hit
AGAATGGGCGCTTGTCGCGTGCCAGATCGGGGTTCAATTCCCCGTCGCGG4030.13640394522180027No Hit
GGGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTG4000.13538853123751887No Hit
GGGTTGTTTGGCCGAGCGGTCTAAGGCGCCTGATTCAAGCTCAGGTATCG3990.13505005990942506No Hit
GCGTAATGGTAACGCGTCTCCTTCCTAAGGAGAAGACTGCGGGTTCGAGT3990.13505005990942506No Hit
CCCCCCATCGTGAGTGCCACTGTAGGCACCATCAATATCTCGTATGCCGT3970.13437311725323747No Hit
GCTCGCGTCGCGTAATGGCAACGCGTCTGACTTCTAATCAGAAGATTATG3850.1303114613161119No Hit
GGTTTCGTGGTCTAGTCGGTTATGGCATCTGCTTGACACGCAGAACGTCC3850.1303114613161119No Hit
GTCTAGTCGGTTATGGCATCTGCTTGACACGCAGAACGTCCCCAGTTCGT3830.1296345186599243No Hit
TAGGAAGAGCGTCAGTCTCATAATCTGAAGGTCGAGAGTTCGAACCTCCC3820.12929604733183053No Hit
GGTTGTTTGGCCGAGCGGTCTAAGGCGCCTGATTCAAGCTCAGGTATCGT3770.12760369069136154No Hit
GCCCAGTTGGTTAAGGCACCGTGCTGATAACGCGGGGATCAGCGGTTCGA3670.12421897741042356No Hit
GGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTGT3610.12218814944186078No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTTTCGCGTGCCAGATCGG3560.12049579280139179No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACTGTAGGCACCATC3540.1198188501452042No Hit
TCCTGGGCGAAATCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC3490.11812649350473521No Hit
GCCTTGTTGCGCAATCGGTAGCGCGTATGACTCTTAATCATAAGGTTAGG3430.11609566553617244No Hit
TCCACAGAGTTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC3280.11101859561476549No Hit
GCGAAAGTGGTTCAGTGGTTAGAATTCATGCTTCCCAAGCATGGGGCCCG3280.11101859561476549No Hit
TCACCGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGCCACTGTAGGCA3270.11068012428667166No Hit
GAAGCGGTGGCTCAATGGTAGAGCTTTCGACTCCAAATCGAAGGGTTGCA3210.1086492963181089No Hit
GCTCGCGTGGCGTAATGGCAACGCGTCTGACTTCTACTCAGAAGATTATG3200.10831082499001508No Hit
CGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCACTGTAGGCACCA3160.1069569396776399No Hit
TTGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCAC3080.10424916905288953No Hit
CTCGCCCCCGGGATACCACTGTAGGCACCATCAATATCTCGTATGCCGTC3070.10391069772479573No Hit
GGGCAACTTGGCCGAGTGGTTAAGGCGAAAGATTGGAAATCTTTTGGGCT3020.10221834108432674No Hit
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2990.10120292710004536Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)