FastQCFastQC Report
Thu 22 Jun 2023
ERR2382723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2382723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513280
Total Bases76.8 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA7255914.136338840399002Illumina PCR Primer Index 6 (96% over 29bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGTC281705.488232543640898No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT208434.060746571072319No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA183843.5816708229426433Illumina PCR Primer Index 6 (100% over 28bp)
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCACC154963.019014962593516No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCGG130742.5471477556109727No Hit
CCCTCACTGGGGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC99351.935590710723192No Hit
TCCTGGGCGAAATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC80511.5685395885286784No Hit
GGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTGT74671.4547615336658355No Hit
GTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA64181.250389650872818Illumina PCR Primer Index 6 (96% over 29bp)
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCGG63751.2420121571072318No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATATCTCGTATGCCGT59671.162523379052369No Hit
ATCCGGGTAGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC56491.1005688902743143No Hit
CGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG52101.0150405236907731No Hit
ATCCTGTCTGTGACGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC51481.0029613466334166No Hit
GTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCGGTTCGATTC49760.9694513715710723No Hit
TCCCGCTAGAGACCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC49480.963996259351621No Hit
ATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGT40900.7968360349127183No Hit
GCCCCCTATGAGGAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC36730.7155938279301746No Hit
GGCAACTTGGCCGAGTGGTTAAGGCGAAAGATTGGAAATCTTTTGGGCTT34510.6723425810473815No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCCTCAATATCTCGTATGCCGT34460.6713684538653367No Hit
ATCCGATTGGAAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC31670.6170121571072319No Hit
CCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACCA30720.5985037406483791No Hit
TTCCCAGCTCGCCCCCCACTGTAGGCACCATCAATATCTCGTATGCCGTC25700.5007013715710723No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA25230.49154457605985036Illumina PCR Primer Index 6 (96% over 29bp)
TGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCCCCACTGTAGGCACC23960.46680174563591026No Hit
GCGCAAGTAGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG23830.46426901496259354No Hit
ATCCCTTAGCTCTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC22900.44615024937655856No Hit
GCGCAATCGGTAGCGCGTATGACTCTTAATCATAAGGTTAGGGGTTCGAG22260.43368142144638405No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATCTCTCGTATGCCGT20560.40056109725685785No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCCTGTAGGCACC20370.3968594139650873No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG18550.3614011845386534No Hit
CTCGCCCCCGGGAGACCACTGTAGGCACCATCAATATCTCGTATGCCGTC18190.3543874688279302No Hit
TGGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTG18000.3506857855361596No Hit
GCCTTGTTGGCGCAATCGGTAGCGCGTATGACTCTTAATCATAAGGTTAG17370.338411783042394No Hit
ATCCGGGTGGGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC16120.3140586034912718No Hit
GGGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTG15570.30334320448877805No Hit
ATCCGGGTAAGACCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC14630.28502961346633415No Hit
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTTGCCATCGTTGGGCCCCCG14450.28152275561097256No Hit
TCCTGAGTGCGAGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC14350.2795745012468828No Hit
TGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCCTGTAGGCACCA14340.2793796758104738No Hit
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG14210.27684694513715713No Hit
ACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTT13690.26671602244389025No Hit
GCGTAATGGCAACGCGTCTGACTTCTAATCAGAAGATTATGGGTTCCCCC13120.2556109725685785No Hit
TCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG11850.2308681421446384No Hit
GGTTGTTTGGCCGAGCGGTCTAAGGCGCCTGATTCAAGCTCAGGTATCGT11820.23028366583541146No Hit
TAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA11610.22619233167082295Illumina PCR Primer Index 6 (100% over 28bp)
CCCCCCATCGTGAGTGCCACTGTAGGCACCATCAATATCTCGTATGCCGT11600.22599750623441398No Hit
ACCTCCCCTGGAGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC11080.21586658354114716No Hit
ATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCT11030.21489245635910223No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGTTCTCACCGTGGAGACCGG10460.20378740648379054No Hit
TCCACAGAATTCGCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC10220.19911159600997505No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCCTCAATATCTCGTATGCCGT10040.1956047381546135No Hit
ATCCTGCTGGTGTCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC9930.19346165835411472No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCCCCACTGTAGGCAC9780.19053927680798005No Hit
ACCTCGGTTGGATCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC9530.1856686408977556No Hit
ACCTCGCATCCTTCACCACTGTAGGCACCATCAATATCTCGTATGCCGTC9290.18099283042394013No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA9210.17943422693266833Illumina PCR Primer Index 6 (100% over 28bp)
TCCGATATAGTGTAACGGCTATCACATCACGCTTTCCCCGTGGAGACCGG9040.1761221945137157No Hit
GCGCAGTTGGTAGCGCGCTTCCCTTGCAAGGAAGAGGTCATCGGTTCGAT9010.1755377182044888No Hit
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8620.16793952618453864Illumina PCR Primer Index 6 (96% over 29bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGTGCCAGATCGGGG8580.16716022443890274No Hit
TCCTGGGCGAAATCGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC8570.16696539900249377No Hit
AAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGCGAAACTCAGGT8450.16462749376558602No Hit
TCCGATGAAACCCTGGTTCGATTCTAGGAGATGGCACCACTGTAGGCACC8250.1607309850374065No Hit
TAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGCGAAACTCAGGT7680.14962593516209477No Hit
GTCTAGTGGTATGATTCTCGCTTTGGGTGCGAGAGGCCCTGGGTTCATTT7540.14689837905236908No Hit
GCGTAGTCGGTAGCGCGCTCCCTTGGCTTGGGAGAGGTCTCCGGTTCGAT7490.1459242518703242No Hit
AGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGC7030.13696228179551123No Hit
CCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA6970.13579332917705736Illumina PCR Primer Index 6 (100% over 28bp)
ATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGC6940.13520885286783044No Hit
AAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGGTGACCATACGCGAAA6920.13481920199501246No Hit
GCGGCGCTACCACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC6820.1328709476309227TruSeq Adapter, Index 6 (96% over 26bp)
TCCTCGGTTGGAACACCACTGTAGGCACCATCAATATCTCGTATGCCGTC6640.1293640897755611No Hit
TTGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCACCACTGTAGGCAC6520.12702618453865339No Hit
ACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTC6410.12488310473815462No Hit
GTCCTGGCGGGGAAGCCACTGTAGGCACCATCAATATCTCGTATGCCGTC6400.12468827930174563No Hit
AGCTTGCCTCGGTAAGTATTATAGCCTGTAGGCACCATCAATATCTCGTA6380.12429862842892768No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCCTCAATCTCTCGTATGCCGT6260.12196072319201996No Hit
AGTGGGTGACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCA6160.12001246882793017No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATCTCTCGTATGCCGT6140.11962281795511222No Hit
GACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA5990.11670043640897756Illumina PCR Primer Index 6 (96% over 29bp)
CGTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCCACTGTAGGCACCA5950.11592113466334164No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTTTCACCGTGGAGACCG5730.11163497506234414No Hit
GCTCGCGTGGCGTAATGGCAACGCGTCTGACTTCTAATCAGAAGATTATG5680.11066084788029926No Hit
TCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCA5570.10851776807980049No Hit
ATAAATCATTTGTATACGACTTAGATGTACCACGGGGTATTGTAAGCAGT5490.10695916458852868No Hit
ACCGAGTAGTGTAGTGGGTGACCATACGCGAAACTCAGGTGCTGCAATCT5400.10520573566084787No Hit
TGGTTGTTTGGCCGAGCGGTCTAAGGCGCCTGATTCAAGCTCAGGTATCG5400.10520573566084787No Hit
AGGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTG5360.10442643391521196No Hit
AGTGTAGTGGGTGACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGG5360.10442643391521196No Hit
GCCCCCTACAGGGCTCCACTGTAGGCACCATCAATATCTCGTATGCCGTC5250.10228335411471322No Hit
AGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5250.10228335411471322Illumina PCR Primer Index 6 (96% over 29bp)
GGCCATCTTAGTATAGTGGTTAGTACACATCGTTGTGGCCGATGAAACCC5180.10091957605985037No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTTGTCGCGTGCCAGATCG5160.10052992518703241No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)