FastQCFastQC Report
Thu 22 Jun 2023
ERR2603019.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2603019.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48034579
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length25-66
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTTCTGGCGCCAAGCGCCCGGC894880.18629912422049125No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGC796410.16579930886872143No Hit
TTCGGTTGGCCTCGGATAGCCGGTC796260.16576808136488508No Hit
CTCGGTTGGCCTCGGATAGCCGGTC643210.13390561828386172No Hit
TTCGCTTCTGGCGCCAAGCGCCCGG636180.13244208927073142No Hit
ACCTCTAGCATCACCAGTATTAGAGGC630330.13122421662111372No Hit
TCTAAACCATTCGTAGACGACCTGCTT619520.12897375451130735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)