FastQCFastQC Report
Thu 22 Jun 2023
ERR2603022.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2603022.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41444539
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length25-66
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTTCTGGCGCCAAGCGCCCGGC2140250.5164130309182592No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGC1709810.4125537504470734No Hit
TTCGCTTCTGGCGCCAAGCGCCCGG1096070.26446668884409597No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC895350.21603570014375115No Hit
CTCGGTTGGCCTCGGATAGCCGGTC663270.16003797267475942No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG633610.15288142063783122No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGG617090.14889537075077613No Hit
TTCGGTTGGCCTCGGATAGCCGGTC603880.14570797855900872No Hit
CCTCGGTTGGCCTCGGATAGCCGGTC545010.13150345332590138No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC529020.12764528518461746No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGC524040.1264436793469943No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG523570.1263302747799897No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG518240.12504421873289506No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGGC510310.12313081827258351No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGCC480820.11601528490882719No Hit
CCGCTTCTGGCGCCAAGCGCCCGGC463070.11173245285705795No Hit
ACCTCTAGCATCACCAGTATTAGAGGC425070.10256357297158017No Hit
TCTCGGTTGGCCTCGGATAGCCGGTC419720.10127269119823No Hit
CGCTTCTGGCGCCAAGCGCCCGGCC416200.10042336337725943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)