FastQCFastQC Report
Fri 23 Jun 2023
ERR2603024.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2603024.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42766719
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length25-66
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGGTTGGCCTCGGATAGCCGGTC1289780.30158497779546756No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC980780.22933253308489715No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGC918870.21485632320777287No Hit
TTCGCTTCTGGCGCCAAGCGCCCGG863360.20187660409488042No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGG711070.16626713870661905No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG615250.14386186604588488No Hit
TCTAAACCATTCGTAGACGACCTGCTT613240.14339187441524331No Hit
CTCGGTTGGCCTCGGATAGCCGGTC582260.13614792380963336No Hit
ACCTCTAGCATCACCAGTATTAGAGGC518350.1212040605686866No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGG427680.10000299531979528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)