FastQCFastQC Report
Fri 23 Jun 2023
ERR2603027.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2603027.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42030480
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length25-66
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGGTTGGCCTCGGATAGCCGGTC1220240.2903226420445353No Hit
TTCGCTTCTGGCGCCAAGCGCCCGGC1050030.249825840675624No Hit
TCGCTTCTGGCGCCAAGCGCCCGGC968150.23034474029323482No Hit
TTCGCTTCTGGCGCCAAGCGCCCGG852300.20278141006241185No Hit
ACCTCTAGCATCACCAGTATTAGAGGC706360.16805898957137771No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGG697840.16603188923847648No Hit
CTCGGTTGGCCTCGGATAGCCGGTC653720.15553474526105818No Hit
TCTAAACCATTCGTAGACGACCTGCTT632690.15053123352386172No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG562790.13390044558139713No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC502210.11948709603126113No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGG454750.10819529065573365No Hit
CTCGCTTCTGGCGCCAAGCGCCCGG424330.1009576859460087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)