FastQCFastQC Report
Fri 23 Jun 2023
ERR2660262_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2660262_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143636526
Total Bases7.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT50409573.5095230582226695No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG37190672.58922093395659No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC30000082.0886108036336104No Hit
TGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT24273421.6899197353185778No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG22240431.548382616828257No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC21821151.5191922700775986No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA21332931.4852023084991628No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG16632761.157975653073091No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA11271810.7847453787625022No Hit
TTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCG7845190.5461834965292881No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG7234580.5036727217977968No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA4916980.3423210054523318No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA4364170.30383427680505165No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGAT4193540.29195498643569257No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCTGTAGGCACCATCAATAG3607300.2511408553559698No Hit
AGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATC3599580.2506033876090821No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATA3573250.2487702884153575No Hit
AAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATC2798340.1948209190188852No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT2740480.19079269572420599No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT2360160.16431475097079415No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT2133870.1485604016905839No Hit
TTTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT2037040.14181908019691314No Hit
TTAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAG1970980.13721997147160186No Hit
TGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATC1843220.12832529798165682No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATAG1803330.12554814922215538No Hit
TTTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGA1797330.12513042817535144No Hit
GTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCG1711080.11912568812754494No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAG1676750.11673562753808178No Hit
ATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGATCG1649890.11486562965188953No Hit
ATTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGA1647070.11466930075989168No Hit
ATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGG1530030.10652095554023633No Hit
TGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGA1526770.10629399377147287No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGA1525680.10621810778130349No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGCTGTAGGCACCATCAATA1466000.10206317576909373No Hit
AAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGAT1441670.10036931692430377No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)