FastQCFastQC Report
Fri 23 Jun 2023
ERR2660263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2660263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133848724
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT60125724.49206523627375No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA57255274.277610446252742No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA41457383.0973309838949232No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA18610651.3904241627286638No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA15603361.165745890861089No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA13196620.9859354355892105No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG10740660.802447694607832No Hit
TGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT10673370.7974203773507769No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC10563690.7892260519420417No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG10290400.7688082256204399No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT8764220.6547854725906838No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATA6611580.4939591355387146No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA6371260.4760045377795309No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCTGTAGGCACCATCAATAG5794490.43291335373507184No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA3671020.27426634265112604No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT3472150.2594085245071145No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAG3252090.242967575843308No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCCTGTAGGCACCATCAATAGA2910070.21741484812361753No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGAT2777810.20753354361450616No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGCTGTAGGCACCATCAATA2652420.19816550511157655No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATC2603670.1945233336703307No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT2473340.18478622179468815No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCTGTAGGCACCATCAATA2372970.1772874577422195No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT2339320.1747734255576467No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA2250140.16811067993446094No Hit
CCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAGA2180680.16292123935376476No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAAT2171130.16220774730732584No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATAGATCGGAAGA2106040.15734479471018342No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC2102740.15709824772031447No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGA2069020.1545789857511081No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAAT2005860.1498602257874345No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT1981750.1480589385372101No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATAG1964490.14676942306898644No Hit
TTAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAG1894370.1415306730903165No Hit
GTCTAGTCGGTTATGGCATCTGCTCTGTAGGCACCATCAATAGATCGGAA1762440.13167402328019204No Hit
GTCTAGTGGTATGATTCTCGCTCTGTAGGCACCATCAATAGATCGGAAGA1743300.13024405073895212No Hit
CCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGATC1712030.12790783123192118No Hit
TCAATATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGAT1639090.12245839564372686No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1598700.11944080991014902No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA1579580.11801233159309012No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1568940.11721740432878537No Hit
TCAATATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGA1520480.11359689913816437No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGTCTGTAGGCACCATCAAT1455210.10872049852339273No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA1400400.1046255771552966No Hit
AGTTTCTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATAGATC1400250.10461437047393893No Hit
TGCTCGTATGGCGCAGTGGTAGCGCAGCAGACTGTAGGCACCATCAATAG1359570.10157511848973623No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)