FastQCFastQC Report
Fri 23 Jun 2023
ERR2660266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2660266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140684593
Total Bases7.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT24172411.7181988080244155No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA19019721.3519405070887898Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA17327951.231687822418479No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA12766790.9074760588744781No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG11933600.8482520896940009No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA9945650.7069466377174649No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATA4196940.29832264574984413No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCTGTAGGCACCATCAATAG4125190.29322258479292046No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG3523920.2504837185689552No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATAG3385030.2406112800141519No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT3250520.23105017619093515No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT3025560.21505979691749189No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT2850800.20263768328917156No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2800070.19903174471990687No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA2730110.19405891873319772No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCTGTAGGCACCATCAATA2685810.19091003092286019No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATCG2685260.19087093637893954No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGAT2562850.18216991252197745No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAG2290050.16277901873732542No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC2206060.15680892647569447No Hit
TGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT2122350.15085873689096857No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATAGATCGGAAGA2037660.14483888793707494No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAAT2004690.14249534773150319No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCCTGTAGGCACCATCAATAGA1975780.1404403963410549No Hit
CCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAGA1818190.12923874329294893No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATA1796440.12769273178335883No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCTGTAGGCACCATCAAT1788140.12710275957510145No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGA1758840.12502008659896396No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1590530.11305644534934965No Hit
CCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGATC1526080.10847527561173667No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)