FastQCFastQC Report
Fri 23 Jun 2023
ERR2660267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2660267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132564691
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATCG15666311.1817860307915629No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT15301801.1542892669662692No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGAT14931491.126354980905134No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAAT14924701.1258427781497262No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG11027160.831832361756118No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCTGTAGGCACCATCAATA9179710.692470214410261No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATA8774110.6618738318486331No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAA7822230.5900688894601656No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGACTGTAGGCACCATCAAT7655220.5774705121139686No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCTGTAGGCACCATCAATAG6544150.493657093049008No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGAT6068030.45774104357848955No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA5881780.4436912993671897No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT5018700.37858497327919693No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCTGTAGGCACCATCAAT4949260.37334677602801486No Hit
TGCTCGTATGGCGCAGTGGTAGCGCAGCAGACTGTAGGCACCATCAATAG4898420.3695116673262566No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATAGATCGGAAGA4859660.3665878118329412No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATAG4835380.3647562532318655No Hit
TTTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAA4583520.3457572273147757No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGA4580240.34550980094692035No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAG4123540.3110586966177894No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA4116870.3105555460465713No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGCTGTAGGCACCATCAATA3965070.2991045330464354No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGACTGTAGGCACCATCAA3364910.2538315425183619No Hit
TTCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAAT3158450.23825725961975802No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAATA3058300.23070245756466176No Hit
CCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAGA2958250.22315519899639039No Hit
TTCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATAG2943310.22202820206475646No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGTCTGTAGGCACCATCAATAGA2812020.2121243582123991No Hit
CCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGACTGTAGGCACCATCAAT2790700.21051608682133918No Hit
TTGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGA2760180.20821381464239225No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGCTGTAGGCACCATCAATAGAT2735960.20638678213341136No Hit
TCCTTGTTAGCTCAGTTGGTAGAGCTTTCGGCCTGTAGGCACCATCAATA2497230.18837821603642557No Hit
CCGTGATAGTTTAATGGTCAGAATGGGCCTGTAGGCACCATCAATAGATC2468830.18623586577816562No Hit
CGTGGAGACCGGGGTTCGACTCCCCGCTTCGGACTGTAGGCACCATCAAT2249620.16969978830939228No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGTCTGTAGGCACCATCAAT2134580.16102176106607452No Hit
CTGCCAGATCGGGGTTCAATTCCCCGTCGCGGACTGTAGGCACCATCAAT2086460.1573918352059524No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2030270.15315314995906415No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGCTGTAGGCACCATCAATAGA2020120.15238748604634095No Hit
TTGGGAGAGGTCTCCGGTTCGATTCCGGACTCGTCCCTGTAGGCACCATC1987120.1498981353941375No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1786060.13473120078407605No Hit
TCCAGAAGATTCCAGGTTCAGGTCCTGGCGGGGACTGTAGGCACCATCAA1760260.13278498118326243No Hit
TTGCGAGAGGCCCTGGGTTCATTTCCCAGCTCGCCCCTGTAGGCACCATC1720720.12980228649271322No Hit
AGAGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAG1687260.1272782358011154No Hit
TCTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA1657180.12500915496419782No Hit
TCGTGGAGACCGGGGTTCGACTCCCCGTATCGGCTGTAGGCACCATCAAT1631230.12305162013314691No Hit
TTGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGCTGTAGGCACCATCAA1630720.12301314835034013No Hit
GTCTAGTCGGTTATGGCATCTGCTCTGTAGGCACCATCAATAGATCGGAA1588090.11979735991690275No Hit
GTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCCTGTAGGCACCATCAATA1580480.119223300569531No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCCTGTAGGCACCATCAATA1566120.11814005586148124No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGCTGTAGGCACCATCAATA1544950.11654309970065861No Hit
GCGCAAGTGGTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATAG1540950.11624136022766424No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1486540.11213694904625847No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA1470580.11093300854901099No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCCTGTAGGCACCATCAATAG1439520.10859000154120979No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGTCTGTAGGCACCATCAATA1428140.10773155274054083No Hit
CGTGGAGACCGGGGTTCGACTCCCCGCTTCGGCTGTAGGCACCATCAATA1371000.10342120436881642No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAG1344920.1014538630048932No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT1337080.10086245363782427No Hit
GATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATC1327760.1001594006657474No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)