FastQCFastQC Report
Fri 23 Jun 2023
ERR2736131.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2736131.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102553513
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length6-70
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAGACGAAGTGCATGAT11012771.0738559487474602No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGA5138820.5010866863234612No Hit
AACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG3891860.3794955322495876No Hit
ATGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG3887240.379045035736611No Hit
TGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG3349720.3266314241229357No Hit
AGACCGAGGGCTTGACC3039320.2963642990952441No Hit
TAGGCGTGAACTCCGGGCTTCC2942480.28692142413492944No Hit
GAGGTTGTAGCTC2928550.2855631088912576No Hit
TAGGCGGGAGAGAGCAAACCTAGTGAACTG2021210.19708832402455098No Hit
AACGGGGCTAAGTGATGTACCGAAGCTGTGGACTC1957370.19086328129978347No Hit
ACTAATAGACCGAGGGCTTGACC1804390.17594619113632898No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAT1691160.16490512616569264No Hit
AATAGACCGAGGGCTTGACC1649380.16083115553535451No Hit
AGTACTAATAGACCGAGGGCTTGACC1587090.15475725341558996No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGA1526860.14888422203537777No Hit
GAGGTTGTAGCTCAGACGGAT1459920.1423568981006043No Hit
AACGATCAGTAGCTGGTCTGAGAGGATGAGC1309440.12768358310650949No Hit
ACGGAGACGAAGTGC1277340.12455350993193183No Hit
ACGGAGACGAAGTGCATGAC1269630.12380170730962672No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAG1219870.11894960633869266No Hit
ACGGGGCTAAGTGATGTACCGAAGCTGTGGACTC1201270.11713591907865702No Hit
CGGGGCGTAGCGCAGCTTGGTAGCGCACCACTTTGG1190690.11610426256192705No Hit
GTACTAATAGACCGAGGGCTTGACC1189650.11600285209147346No Hit
TAGGCGGGAGAGAGCAAACCTAGTGAACT1186990.11574347531127481No Hit
ATTTGTCGTCTCAATTAAGGAGGCAATTCTGTGCAAACC1148470.11198738750178164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)