FastQCFastQC Report
Fri 23 Jun 2023
ERR2736133.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2736133.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96384647
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length6-70
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAGACGAAGTGCATGAT20313942.107590848986561No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGA8572210.8893750474595815No Hit
TAGGCGGGAGAGAGCAAACCTAGTGAACTG3843040.3987191030538297No Hit
GAGGTTGTAGCTC3692840.3831357083249991No Hit
AACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG3273900.3396702796452634No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGA2732400.28348913286988536No Hit
AGACCGAGGGCTTGACC2643200.2742345469190752No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAG2502740.25966168657545635No Hit
TAGGCGTGAACTCCGGGCTTCC2343990.24319122110806712No Hit
AGGCGGGAGAGAGCAAACCTAGTGAACTG2250690.23351125620660312No Hit
ATGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG2115240.21945818819049057No Hit
TAGGCGGGAGAGAGCAAACCTAGTGAACT2107370.21864166810716232No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAT1925350.1997569177174037No Hit
ACGGAGACGAAGTGCATGAC1864070.1933990586695825No Hit
TGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG1836430.19053138203639425No Hit
AACGGGGCTAAGTGATGTACCGAAGCTGTGGACTC1566910.16256842233390137No Hit
ACTAATAGACCGAGGGCTTGACC1535370.1592961169427741No Hit
AGTACTAATAGACCGAGGGCTTGACC1501320.1557633966330758No Hit
AAGTGATGTACCGAAGCTGTGGAC1473020.15282724436392864No Hit
AATAGACCGAGGGCTTGACC1430040.1483680279495136No Hit
ACGGAGACGAAGTGC1409370.14622349553243683No Hit
TCCTCGGTAGCTCAGTGGTAGAGCGGTCGGCTG1360250.14112724820167677No Hit
GTACTAATAGACCGAGGGCTTGACC1291240.13396739420542778No Hit
GAGGTTGTAGCTCAGACGGAT1253880.1300912582062992No Hit
AGTTCAGATTGCAGGCTG1192130.12368463620559818No Hit
AGGCGGGAGAGAGCAAACCTAGTGAACT1160210.12037290544831274No Hit
AAGTGATGTACCGAAGCTGTGGACTC1104390.11458152666160618No Hit
ACGGGGCTAAGTGATGTACCGAAGCTGTGGACTC1092280.11332510249272376No Hit
ACAGCGGCGACGATACTCTGGGGG1069110.11092119266671176No Hit
AGCGGCGACGATACTCTGGGGG1041530.10805974109133792No Hit
CGGGGCGTAGCGCAGCTTGGTAGCGCACCACTTTGG990090.10272279152508594No Hit
AGGCGTGAACTCCGGGCTTCC976090.10127027803504846No Hit
AAACGACCAGTAAGACGGTGGGGG972940.10094346249979003No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)