FastQCFastQC Report
Fri 23 Jun 2023
ERR2736134.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2736134.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105335418
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length6-70
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAGACGAAGTGCATGAT22798642.164385012456114No Hit
TAGGCGTGAACTCCGGGCTTCC9113940.8652303444602081No Hit
GAGGTTGTAGCTC7969220.7565565458713991No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGA5981250.5678289518915661No Hit
CGCGGGATAGAGCAGTCTGGT5835890.5540292250038823No Hit
AGTTCAGATTGCAGGCTG5347940.5077057747091296No Hit
AGCAATCGTCAAGAGGTATTAACTATG4917030.4667974071171389No Hit
TCAGCAATCGTCAAGAGGTATTAACTATG4527590.4298259869249296No Hit
TTTCAGCAATCGTCAAGAGGTATTAACTATG4478660.42518082569340543No Hit
GAGGTTGTAGCTCAGACGGAT4148450.3938323954816413No Hit
TTCAGCAATCGTCAAGAGGTATTAACTATG3696160.3508943212244148No Hit
ACGGAGACGAAGTGC3626310.3442631233494512No Hit
TTTTCAGCAATCGTCAAGAGGTATTAACTATG3370260.31995506012991753No Hit
AGCTGGTCTGAGAGGATG3317960.3149899685213192No Hit
AGACCGAGGGCTTGACC3070480.2914954967948198No Hit
AAGTGATGTACCGAAGCTGTGGAC2842970.269896873623267No Hit
TAGGCGTGAACTCCGGGCTTC2674630.25391554434235974No Hit
AGGCGTGAACTCCGGGCTTCC2574310.24439168219753016No Hit
AAGTGATGTACCGAAGCTGTGGACTC2560950.24312335286883277No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAT2351950.22328197340043782No Hit
ACTAATAGACCGAGGGCTTGACC2202160.20906168521588817No Hit
ACGGAGACGAAGTGCATGAC2114530.20074254606366115No Hit
AGTACTAATAGACCGAGGGCTTGACC1967570.18679092344798973No Hit
AATAGACCGAGGGCTTGACC1790530.16998366114614932No Hit
TAAGGATTAGAGGT1783050.16927354861780677No Hit
AGTGATGTACCGAAGCTGTGGACTC1774710.16848179213567083No Hit
TAGGCGTGAACTCCGGGCTTCT1763520.16741947138805677No Hit
AGCTGTATCCCGAGTAGGTCGGAGCTCG1738170.16501287344775145No Hit
GTACTAATAGACCGAGGGCTTGACC1650850.15672316409282203No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGA1581660.1501546232056534No Hit
TAGGCGGGAGAGAGCAAACCTAGTGAACTG1564660.14854073109578395No Hit
AGTGATGTACCGAAGCTGTGGAC1512260.14356614600418635No Hit
GGGGCTGTAGCTC1425000.13528213273905648No Hit
ATATCCGGTGTAGGCGTAAGAGT1413380.13417899001454572No Hit
TTGACTTTTTTCTCTGGAGGAAATCCACCATG1397650.13268566513876653No Hit
AGTTCAGATTGCAGGCT1317160.12504436067268468No Hit
AGTAGCTGGTCTGAGAGGATG1280120.12152797456976912No Hit
ACGGTCCTAAGGTAGCGA1210930.11495943368260046No Hit
AGGAGTACTAATAGACCGAGGGCTTGACC1185060.11250346963069914No Hit
ATCAGTAGCTGGTCTGAGAGGATG1138120.10804722871085963No Hit
AGGAAGCTTGACTGTGAGACTG1133320.10759154152689648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)