FastQCFastQC Report
Thu 29 Jun 2023
ERR2812328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89507736
Total Bases9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG69489507.763518898522917TruSeq Adapter, Index 11 (100% over 49bp)
AGGCCCCGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT39888754.456458378078069No Hit
AAGGCCCCGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG15836171.7692515426822997No Hit
TCGTCGCCGAATCCCGGGGCCGAGGGAGAAGATCGGAAGAGCACACGTCT13777001.5391965673224044Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGCCTGAGCGTCGCTTAGATCGGAAGAGCACACG11684691.3054391186924892No Hit
TCGTCGCCGAATCCCGGGGCCGAGGGAGAGAGATCGGAAGAGCACACGTC11291401.2614998998522318No Hit
TCGTCGCCGAATCCCGGGGCCGAGGGAGAGGAGATCGGAAGAGCACACGT9257581.0342770819273097No Hit
ACGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG8822180.9856332418015802Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCT8114470.9065663329927148Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAACAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA7711980.8615992700340448Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTCGCCGAATCCCGGGGCCGAGGGAGAAGATCGGAAGAGCACACGTC5833100.6516866877294271No Hit
TCGTCGCCGAATCCCGGGGCCGAGGGAGAGGACAGATCGGAAGAGCACAC5529520.6177700662655572No Hit
TCGTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG5513340.615962401283393Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGCCTGAGCGTCGCTAGATCGGAAGAGCACACGT5405560.6039209839918194No Hit
TCCCGGGGCTACGCCTGCCTGAGCGTCGCTTAGATCGGAAGAGCACACGT5241860.5856320620152877No Hit
ATCGTCGCCGAATCCCGGGGCCGAGGGAGAGAGATCGGAAGAGCACACGT4700230.5251199739874998No Hit
TCGTCGCCGAATCCCGGGGCCGAGGGAGAGGAAGATCGGAAGAGCACACG4624190.5166246189044487No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT4234140.47304737994937107No Hit
ATCGTCGCCGAATCCCGGGGCCGAGGGAGAGGAGATCGGAAGAGCACACG3579300.39988722315577285No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACAGATCGGAAGAGCACACGTCTGAA2935410.32795042430745874Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGA2792570.3119920271472401Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGGGCTACGCCTGCCTGAGCGTCGCTAGATCGGAAGAGCACACGTC2603610.2908810027325459No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2501110.27942947858719164No Hit
CTAAATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGA2388280.26682386425235916Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGCATTCAGCTTACACCTGAAAGATAAGATCGGAAGAGCACACGTCTG2221450.2481852518311937Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCGCGGGCGAGGCCGCGGGGGGCGCCGGGGGGAGATCGGAAGAGC2136220.23866316985159808No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2064550.23065604072479276Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAATCATTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAA1849530.206633536122509Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1803740.20151777718967218No Hit
CCGCCGGAATACTCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC1781000.1989772146622053No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1715090.1916136053312755Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCCGGCGGGCCAAGGTCGGCCGGCGCGGGAGATCGGAAGAGCACACGT1680260.1877223215655907No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGGCAGATCGGAAGAGCAC1619360.1809184403904485No Hit
GATTCAACCCGGCGGGCCAAGGTCGGCCGGCAGATCGGAAGAGCACACGT1536310.1716399127780419No Hit
CGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGAAGATCGGAAGAGCACA1510690.16877759035263723No Hit
AACCCGGCGGGCCAAGGTCGGCCGGCAGATCGGAAGAGCACACGTCTGAA1485890.1660068801203954Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATTAACAGTCCTACGTGATCTGAGATCGGAAGAGCACACGTCTGAAC1480890.16544826918647568Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTCGCCGAATCCCGGGGCCGAGGGAGAGGACCAGATCGGAAGAGCACA1458740.16297362274921132No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1351570.15100035599157596Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA1318970.14735821270241936Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATTAACAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTCT1262580.14105819858967275Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA1231820.1376216241241986Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTCGCCGAATCCCGGGGCCGAGGGAGAGGAAGATCGGAAGAGCACAC1218410.13612342959942592No Hit
CGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1210290.1352162454427403Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCCCGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1143900.1277990094621542No Hit
AGTCGGCCCGGAGGATTCAACCCGGCGGGCCAGATCGGAAGAGCACACGT1042110.1164268080694165No Hit
ATTCAGCTTACACCTGAAAGATAAGATCGGAAGAGCACACGTCTGAACTC1035710.11571178607399923Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCATCTTAGCCTCAACGATTAATTTTAACAGATCGGAAGAGCACACGTCT1025050.11452082756288239Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT993220.11096471035754944No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC965140.10782755135265627TruSeq Adapter, Index 11 (100% over 50bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC965110.10782419968705274No Hit
CGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGACAGATCGGAAGAGCAC950670.10621093130989258No Hit
CGGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTG909000.10155546778660561Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)