FastQCFastQC Report
Mon 24 Jul 2023
ERR2812329.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812329.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117636574
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG43745753.71872016605992Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAACAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA16424041.3961678278729879Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGAG8990270.7642410599275018No Hit
AGCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGA7190120.6112146720627889No Hit
CGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA5974220.5078539604528095Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGA5867910.4988168050524831Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATTAACAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTCT5745950.4884492810883799Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC5597870.47586135924019685No Hit
GGTAATCTATGAAATTAGTATTCCCGTGAGATCGGAAGAGCACACGTCTG4872970.41423936742666445Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCTATTCTCAAACTTTCAATGGGTAAGACGAGATCGGAAGAGCACACG4711910.4005480472425183No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA4581480.3894605091100324Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA4143090.3521940378848503Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA3950140.3357918261033342Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGAAGATCGGAAGAGCACA3750020.31878011000218354No Hit
CTAAATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGA3636760.30915215194893386Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT3194810.2715830537533335No Hit
AACGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCT3194240.2715345994350363Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT2806110.2385406089776127Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACG2557500.21740687551815305No Hit
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACA2555600.2172453611238287No Hit
AGCCTTCAGCTAACAACAAGTATTGAAGGTGAGATCGGAAGAGCACACGT2401300.2041286921531734No Hit
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCAC2390780.20323441245407234No Hit
CTTCAAAGCATTCAGCTTACACCTGAAAGATAAGATCGGAAGAGCACACG2356310.20030420131072502No Hit
CGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGACAGATCGGAAGAGCAC2310100.19637600122560522No Hit
CGGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTG2253450.1915603220474612Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2214920.18828498014571557Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC2185140.1857534545336215Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAAGCATTCAGCTTACACCTGAAAGATAAGATCGGAAGAGCACACGT2148820.18266597937474785No Hit
AATGAAAGAACAACAGTGAGCTCAATAGAGATCGGAAGAGCACACGTCTG2146100.18243475876813617Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGGCGCGGGAGGTGTGGCGTACAGATCGGAAGAGCACACGTCTGAA2116750.17993978641370498Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGGTCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC2067920.17578886647956954No Hit
CGATTAACAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA2063240.17539103102407588Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2026480.1722661525317798No Hit
AACCTATTCTCAAACTTTCAATGGGTAAGACAGATCGGAAGAGCACACGT1992030.16933764153995168No Hit
CGCGACCTCAGGTCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1974040.16780835524842808No Hit
CGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1942070.16509066304498124No Hit
ACGATTAACAGTCCTACGTGATCTGAGATCGGAAGAGCACACGTCTGAAC1933430.1643561975886853Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT1790980.15224686839315807No Hit
AGCCTTCAGCTAACAACAAGTATTGAAGGTAGATCGGAAGAGCACACGTC1714930.14578204224138658No Hit
AACGATTAACAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTC1678390.1426758654158017No Hit
CCGCCGGAATACTCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC1614240.13722262941795635No Hit
GCGACCTCAGGTCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1613280.1371410221450346No Hit
GCCTTCAGCTAACAACAAGTATTGAAGGAGATCGGAAGAGCACACGTCTG1572720.1336931148640898Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGAAAGAACAACAGTGAGCTCAATAGCAGATCGGAAGAGCACACGTCT1566730.13318391948408834Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCACCTCTAATGAAAGAACAACAGTGAGAGATCGGAAGAGCACACGTCT1561370.1327282788769418Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGTCTGA1526020.12972326106674953Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCAGCTTACACCTGAAAGATAAGATCGGAAGAGCACACGTCTGAACTC1487270.12642921749829267Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGAATTTTTTGAGGCTTCGCTTCGGCGGAGATCGGAAGAGCACACGTC1477670.12561314476907498No Hit
CGCGACCTCAGGTCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1451340.1233748952940435No Hit
AAATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGAAC1419560.12067335453002909Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGT1389600.11812652755426216No Hit
CGGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACAC1380020.11731215497656367No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT1356750.11533402868397033No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1321130.11230605882826883No Hit
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT1274440.10833705510668815Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT1269450.10791286730264688Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGACCTCAGGTCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA1227220.10432299736984858No Hit
TTGTCCCAGTATAGGCGATAGAAAAGACTACCAGATCGGAAGAGCACACG1213100.1031226903972909No Hit
TGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCACAC1203370.10229556668319838No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC1195010.10158490334817129Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGCGGTCGGTGCGGAGCGCCGCTCGTGGTCGGGAAGATCGGAAGAGCAC1191700.1013035282717431No Hit
AGCCTTCAGCTAACAACAAGTATTGAAGGAGATCGGAAGAGCACACGTCT1190330.10118706789267766Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)