FastQCFastQC Report
Sat 24 Jun 2023
ERR2812330.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812330.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144612421
Total Bases8.1 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG34191102.364326643836493Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGAG17802821.231071292278552No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA17202621.1895672502433245Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGA10850920.7503449513510323No Hit
ACGATTAACAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA10060680.6956995761795594Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGAAGATCGGAAGAGCACA8415250.5819175103914483No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA7352920.5084570155975745Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA7300420.5048266220506743Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACA7101240.49105325468550176No Hit
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCAC6471460.44750374520042097No Hit
ACGATTAACAGTCCTACGTGATCTGAGATCGGAAGAGCACACGTCTGAAC6102160.42196651973622656Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGACAGATCGGAAGAGCAC5693800.3937282814731385No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGA5269680.3644002336424476Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATTAACAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTCT5175860.35791254749825396Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT4956460.3427409599898753No Hit
CGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA4633670.32041991745646803Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTAATCTATGAAATTAGTATTCCCGTGAGATCGGAAGAGCACACGTCTG4414880.3052905116635866Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCACAC4224900.2921533275485375No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC4106570.28397076624559103No Hit
AACCTATTCTCAAACTTTCAATGGGTAAGACGAGATCGGAAGAGCACACG4075450.2818188072516952No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT3922760.2712602398102442Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT3875950.2680233117734748Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA3643370.25194032260894106Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGTCTGA3322000.22971747357718328Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC3196600.22104601927658757Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCAC2990790.20681418506920649No Hit
TTCAAAGCATTCAGCTTACACCTGAAAGATAAGATCGGAAGAGCACACGT2985430.20644353917565628No Hit
CGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGAGATCGGAAGAGCACAC2725510.18846997935260346No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC2671930.18476490342416713No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT2629000.18179627875810198No Hit
CTTCAAAGCATTCAGCTTACACCTGAAAGATAAGATCGGAAGAGCACACG2584720.17873430111511654No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT2584400.17871217300206876No Hit
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT2400000.16596084785829013Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC2317320.16024349664957202No Hit
AAAGCATTCAGCTTACACCTGAAAGATAAGATCGGAAGAGCACACGTCTG2288820.15827271158125483Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT2106460.14566245315815576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT2030150.14038558969979487No Hit
AACGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCT1967980.1360865122367324Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTG1964940.13587629516277858Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACG1939530.13411918468607895No Hit
GCGACCTCAGGTCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1886010.13041825777883906No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1878070.12986920397384122Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG1854000.1282047549705291No Hit
AGCCTTCAGCTAACAACAAGTATTGAAGGTAGATCGGAAGAGCACACGTC1852380.12809273139822477No Hit
TGAGAACGTTGGGGTTAAGAGCAATTCGATGGGAGATCGGAAGAGCACAC1797290.12428323843634427No Hit
CTAAATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGA1745040.12067013247776275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT1737090.12012038716923217Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGTCTGAACT1728200.11950564052862374Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC1691420.11696229053519545No Hit
GCGCCGAGGCGCCTCGGTGGGCTCGCGATAGATCGGAAGAGCACACGTCT1681030.11624381836467561Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACAC1679320.11612557126057657No Hit
GGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACAC1657210.11459665694968207No Hit
GTGGAATTTTTTGAGGCTTCGCTTCGGCGGAGATCGGAAGAGCACACGTC1650920.1141617012275868No Hit
GCGCCGACCTCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACA1632460.11288518570614346No Hit
AGCCTTCAGCTAACAACAAGTATTGAAGGTGAGATCGGAAGAGCACACGT1595450.11032593113146207No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1584230.10955006416772457Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGAAGATCGGAAGAGCACAC1568780.10848169120963683No Hit
TTGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCACA1541810.1066167061818293No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACAGATCGGAAGAGCACACGTCTGAA1527170.10560434500989373Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACA1516500.10486651074045708No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG1488630.10293929039470269No Hit
GCAGTTTTACCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACA1466850.1014331957003887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)