FastQCFastQC Report
Thu 22 Jun 2023
ERR2812334_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812334_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50822663
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG18110153.563400445978205TruSeq Adapter, Index 1 (100% over 49bp)
AAGGCCCCGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG16739363.293680222935189No Hit
CTCCCGGGGCTACGCCTGCCTGAGCGTCGCTTAGATCGGAAGAGCACACG12125412.385827362096315No Hit
ATCGTCGCCGAATCCCGGGGCCGAGGGAGAGAGATCGGAAGAGCACACGT3963030.7797761404198753No Hit
ATCGTCGCCGAATCCCGGGGCCGAGGGAGAGGAGATCGGAAGAGCACACG3523170.6932281372190198No Hit
TCGTCGCCGAATCCCGGGGCCGAGGGAGAGGAGATCGGAAGAGCACACGT2943990.5792671666968731No Hit
AGCCTTCAGCTAACAACAAGTATTGAAGGTGAGATCGGAAGAGCACACGT2932010.5769099505864146No Hit
CTCCCGGGGCTACGCCTGCCTGAGCGTCGCTAGATCGGAAGAGCACACGT2696480.5305664522144382No Hit
CCGCCGGAATACTCCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC2179140.4287732817148916No Hit
TGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTC2108150.4148051037782101No Hit
TCCCGGGGCTACGCCTGCCTGAGCGTCGCTTAGATCGGAAGAGCACACGT2098070.41282173663351723No Hit
ACGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG2022890.398029123346016Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTCGCCGAATCCCGGGGCCGAGGGAGAGGAAGATCGGAAGAGCACACG1773890.3490352325693756No Hit
CTCGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1759690.34624120345681214No Hit
GTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGT1689630.3324560147507422No Hit
TCGTCGCCGAATCCCGGGGCCGAGGGAGAGGACAGATCGGAAGAGCACAC1624820.3197038297658665No Hit
AGAGCCACATTAGGAATTGGTGCTTAAGAAGATCGGAAGAGCACACGTCT1613160.3174095776917475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCTCCTGCTGAAGAACGAGAAGTAGGAGATCGGAAGAGCACACGTCT1520260.29913033089194874Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG1498030.2947562979924921Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA1339510.26356548849083333Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAAC1165690.2293642110016943Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTCGCCGAATCCCGGGGCCGAGGGAGAGGAAGATCGGAAGAGCACAC1152820.2268318761651667No Hit
AGGCCCCGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1133560.22304222822798564No Hit
AACCCGGCGGGCCAAGGTCGGCCGGCGCGGGAGATCGGAAGAGCACACGT938270.1846164574256961No Hit
AGCATTGTGATTCATGATACGTGCAGGAAAGATCGGAAGAGCACACGTCT912570.1795596582571834Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACG900460.1771768630069621No Hit
GCGACCTCAGGTCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG877550.17266903153028404No Hit
ATCGTCGCCGAATCCCGGGGCCGAGGGAGAAGATCGGAAGAGCACACGTC868190.1708273334673549No Hit
GAATCACTTCTGGAAAAGTGCTGCAAAACAGATCGGAAGAGCACACGTCT864290.17005995927446776Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTTCAGCTAACAACAAGTATTGAAGGTAGATCGGAAGAGCACACGTC863660.16993599882792446No Hit
AGTGTGAAGCAGCAGTATTTTTGTGGAATAGATCGGAAGAGCACACGTCT854980.16822809934221666Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG852730.16778538346170485No Hit
CGGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTG850570.16736037621641353Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT847180.16669335095644242No Hit
GGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTCT839810.16524321049449928Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACT738350.14527967572262004Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGAAGATCGGAAGAGCACA736890.14499240230682128No Hit
GCGACCTCAGGTCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC728100.14326285893362178No Hit
GAAAAGTACATAGAAGATAAGGAAGTGTGAGATCGGAAGAGCACACGTCT714340.14055540537102512Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGA706950.13910132965681077Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC699630.1376610273255457Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG673820.13258258426954134No Hit
AGGTAATCTATGAAATTAGTATTCCCGTGAGATCGGAAGAGCACACGTCT666150.13107341502352995Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCTTGAAACTGGAAAGAAAATGACAGCAGATCGGAAGAGCACACGTCT650810.12805507653150722Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGACAAACTGTGATCTTCTCCATGACCAAGATCGGAAGAGCACACGTCT618340.12166619446918789Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTCGCCGAATCCCGGGGCCGAGGGAGAGAGATCGGAAGAGCACACGTC610740.12017079860612578No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG609530.1199327158437172Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAACAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTCT588040.11570428727829551Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC586780.1154563663852089TruSeq Adapter, Index 1 (100% over 50bp)
CAGGAGACCACACCTATAAATGACAACGTAGATCGGAAGAGCACACGTCT572850.1127154631783069Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGCGCGGGAGGTGTGGCGTACGGAAGCCAGATCGGAAGAGCACACGT550380.10829420725159561No Hit
CGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGACAGATCGGAAGAGCAC528390.1039673973793935No Hit
TACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAA513520.10104153731574435Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGA512270.10079558404879334Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)