FastQCFastQC Report
Fri 23 Jun 2023
ERR2812335.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812335.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89731578
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACGT10702831.1927607023694602No Hit
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCAC9979421.1121413690061261No Hit
AGCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGA8474060.9443788005154663No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGA8367570.9325111835211458Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACA8001440.8917083794068572No Hit
CTAAATCATTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAA6330660.7055108291977212Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACAC6294690.7015022069488179No Hit
GCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTCTGAACT6208340.6918790617947229Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTG6158780.6863559225493616Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG5912310.6588884461610605Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTC5833600.6501167292522149No Hit
GGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACGTC5104660.5688811134024635No Hit
AAATCATTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAACT4910080.5471964395856272Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACA4564670.5087027445343711No Hit
CTAAATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGA4462180.497280901490443Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGGGGCGCGGGAGGTGTGGCGTACAGATCGGAAGAGCACACGTCTGAA4334430.4830439959497871Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGAAC4235270.4719932597195605Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGAA3873970.43172872765037074Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATTCAACCCGGCGGGCCAAGGTCGGCCGGCAGATCGGAAGAGCACACGT3781320.4214034885244078No Hit
CGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT3520540.392341255828578Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACACG3397450.37862367694012916No Hit
GCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGAG3234790.36049627924742395No Hit
GTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGT3224200.35931609271376014No Hit
GCGCCGACCTCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACA3117920.3474718788518352No Hit
CGGTGGGGCGCGGGAGGTGTGGCGTACAGATCGGAAGAGCACACGTCTGA2931710.3267199870261949Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC2913830.3247273774679411No Hit
TGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGAAC2739940.3053484694095093Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATTCAACCCGGCGGGCCAAGGTCGGCCGGCGCGGGAGATCGGAAGAGCA2679260.29858607858205727No Hit
CGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTCTGAAC2609980.29086527376126164Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTCTGAA2539990.2830653440642713Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2480910.2764812628169762No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2468630.2751127367892717No Hit
GGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTCT2411590.2687560002566767Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC2398450.26729163282963775No Hit
GGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACAC2349410.2618264441978274No Hit
TGGGGCGCGGGAGGTGTGGCGTACGGAAGCCAGATCGGAAGAGCACACGT2207160.24597360808699922No Hit
AATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGAACT2200500.24523139445959594Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2149720.23957229415936493No Hit
AGCGCCGACCTCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCAC1961190.21856185344249712No Hit
TAAATCATTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAAC1891200.21076192374550684Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGCGCGGGAGGTGTGGCGTACGGAAGCCAGATCGGAAGAGCACACG1693360.18871394415910084No Hit
AATCATTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAACTC1649950.18387618236246775Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGCTGCCGGCGGGCAGGTGGCCCGGCGCCAGATCGGAAGAGCACACGT1624930.18108786630276355No Hit
ATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGAACTC1606550.17903953500071068Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1602880.1786305374012257No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1519290.16931497627290137No Hit
ACGATTAACAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA1491100.16617338435751125Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACG1423870.1586810386862917No Hit
CGGTGGGGCGCGGGAGGTGTGGCGTACGGAGATCGGAAGAGCACACGTCT1271560.14170708109022667Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGAA1259040.14031180862549858Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1249890.13929210071397607Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1241020.138303596978981No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACGGAGATCGGAAGAGCACACGTCTG1222930.13628758428833157Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1171470.13055270241653388Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCACAC1142980.12737767745486434No Hit
AGTGCTCCTGCTGAAGAACGAGAAGTAGGAGATCGGAAGAGCACACGTCT1138940.12692744576496803Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGGGTGGGGTCCGCGCAGTCGGCCCGGAGAGATCGGAAGAGCACACG1103780.12300909274101922No Hit
CGCGGGAGGTGTGGCGTACGGAAGCCAGATCGGAAGAGCACACGTCTGAA1089780.12144888391464596Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGCGCGGGAGGTGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCT1083670.12076796420542163Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACG1044200.11636928974992505No Hit
GGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTCTGA1025940.11433433166638393Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGTGAAGCAGCAGTATTTTTGTGGAATAGATCGGAAGAGCACACGTCT1005970.11210880521905009Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCGCGGGAGGTGTGGCGTACGGAAGCCAGATCGGAAGAGCACACGTC987270.11002481200096581No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACAC938600.10460085745956679No Hit
GCGGGAGGTGTGGCGTACGGAAGCCAGATCGGAAGAGCACACGTCTGAAC901660.10048413502769338Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGCGCGGGAGGTGTGGCGTACAGATCGGAAGAGCACACGTCTGAAC897620.1000339033377971Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCCGGCGGGCCAAGGTCGGCCGGCGCGGGAGATCGGAAGAGCACACGT897440.10001384351002944No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)