FastQCFastQC Report
Wed 19 Jul 2023
ERR2812336.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812336.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225663389
Total Bases12.5 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGA65944042.9222303313011047No Hit
GATTCAACCCGGCGGGCCAAGGTCGGCCGGCGCGGGAGATCGGAAGAGCA41871381.855479534608957No Hit
GCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGAG31234391.3841141949702793No Hit
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCAC24296051.076650054209724No Hit
ACGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG16868780.747519572171275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCAC16004140.7092040969038181No Hit
AGCCTTCAGCTAACAACAAGTATTGAAGGTGAGATCGGAAGAGCACACGT13381870.5930013751588211No Hit
AACCCGGCGGGCCAAGGTCGGCCGGCGCGGGAGATCGGAAGAGCACACGT13024660.5771720462817298No Hit
GGATTCAACCCGGCGGGCCAAGGTCGGCCGGCGCGGGAGATCGGAAGAGC12291000.5446607912105761No Hit
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACA11724500.5195570292529818No Hit
AGCCTTCAGCTAACAACAAGTATTGAAGGTAGATCGGAAGAGCACACGTC10608620.4701081574202539No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGA8732320.38696219349962874Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCA8155350.36139446616216514No Hit
GGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACA7609100.3371880584493039No Hit
GGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACAC6863100.3041299712112362No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACG5711040.253077826461252No Hit
GAAAAGTACATAGAAGATAAGGAAGTGTGAGATCGGAAGAGCACACGTCT5656080.2506423405703616Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG5226220.2315936148596971Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCACAC5086810.22541582941484584No Hit
CTGCTTGAAACTGGAAAGAAAATGACAGCAGATCGGAAGAGCACACGTCT5059470.22420429039998158Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACAC5050080.22378818391316457No Hit
TTGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCACA5013820.2221813658927191No Hit
CTAAATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGA4991060.22117278403542898Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA4916780.2178811557243785Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGCCACATTAGGAATTGGTGCTTAAGAAGATCGGAAGAGCACACGTCT4694060.20801158844601064Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG4585540.20320265597003864Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA4584930.203175624558222Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATCACTTCTGGAAAAGTGCTGCAAAACAGATCGGAAGAGCACACGTCT4325120.19166245881382205Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGAGGCGCCTCGGTGGGCTCGCGATAGATCGGAAGAGCACACGTC4258580.18871381923631395No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA4252320.18843641491176932Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTAATCTATGAAATTAGTATTCCCGTGAGATCGGAAGAGCACACGTCTG4105490.18192982114613196Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTAATCTATGAAATTAGTATTCCCGTGAGATCGGAAGAGCACACGTCT4075160.1805857838995762Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGATTCAACCCGGCGGGCCAAGGTCGGCCGGCAGATCGGAAGAGCACACG3954370.17523312122198076No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3951760.1751174622304374No Hit
CTTTGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCA3912920.17339631463214444No Hit
GCGCCGAGGCGCCTCGGTGGGCTCGCGATAGATCGGAAGAGCACACGTCT3899910.1728197922260221Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATTCAACCCGGCGGGCCAAGGTCGGCCGGCAGATCGGAAGAGCACACGT3876040.17176202206198365No Hit
AACCTATTCTCAAACTTTCAATGGGTAAGACGAGATCGGAAGAGCACACG3825980.1695436737414238No Hit
ACGATTAACAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA3754860.1663920770063415Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTG3683000.16320768806676036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCCAGATCGGAAGAGCACAC3641740.16137930109699805No Hit
ATCTGAGCATTGTGATTCATGATACGTGAGATCGGAAGAGCACACGTCTG3622220.16051429591886524Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTC3528600.15636563891185734No Hit
AGTGCTCCTGCTGAAGAACGAGAAGTAGGAGATCGGAAGAGCACACGTCT3453820.15305185370587518Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGACAGATCGGAAGAGCAC3181900.14100204796622992No Hit
GCCTTCAGCTAACAACAAGTATTGAAGGTGAGATCGGAAGAGCACACGTC3147400.13947322221594394No Hit
GCGACCTCAGGTCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA3030070.13427388525127573No Hit
GTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGT3026370.1341099242287813No Hit
GATTCAACCCGGCGGGCCAAGGTCGGCCGGCGCGGGCAGATCGGAAGAGC2758790.12225244033714304No Hit
AGCATTGTGATTCATGATACGTGCAGGAAAGATCGGAAGAGCACACGTCT2753950.12203796159420437Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGTGAAGCAGCAGTATTTTTGTGGAATAGATCGGAAGAGCACACGTCT2681230.11881546279534072Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGAAGATCGGAAGAGCACA2642370.11709342892125049No Hit
AGAGCCACATTAGGAATTGGTGCTTAAGACAGATCGGAAGAGCACACGTC2642320.11709121323175732No Hit
CGGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCCAGATCGGAAGAGCACA2523130.11180945261794327No Hit
AAGTACATAGAAGATAAGGAAGTGTGTAAAGATCGGAAGAGCACACGTCT2403940.1065276920041292Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAACAGTCCTACGTGATCTGAGATCGGAAGAGCACACGTCTGAAC2393740.10607569134752293Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)