FastQCFastQC Report
Fri 23 Jun 2023
ERR2812340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176588839
Total Bases9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG62651853.5478940999210034Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA33868981.917957000668655Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT33677281.9071012749565672Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA28334661.6045555404551926Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC26554341.503738296846722Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGACAGATCGGAAGAGCAC21447941.2145693986922923No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG20282401.1485663598479177Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT17319310.9807703645415552Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGTCTGAACT17142710.9707697325084061Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGCGCCCGGGGTCCGCGGCGACAGATCGGAAGAGCACACGTCTGAACT15975700.9046834494449562Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG13717950.7768299558275028Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA11895300.6736156184819811Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTGCGCCCCGACCGCGCGGCGCCGCCGGGCCGGAGATCGGAAGAGCAC11588370.6562345653113445No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA11171060.6326028339763873Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA10317940.5842917399779722Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG7938890.4495691825687806Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGAAGATCGGAAGAGCACA7883940.4464574343795306No Hit
AGTGCGCCCCGACCGCGCGGCGCCGCCGGGCCGGAGATCGGAAGAGCACA7402430.41919013919107306No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT7398690.41897834777655457Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACAC7304950.4136699715206803No Hit
CGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACGT7248350.4104647859426722No Hit
CGGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTG6285030.35591320694961925Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGA5984440.33889117986669587Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACA5780460.3273400534673655No Hit
CTAAATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGA5703860.32300229348016724Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGAGGCGCCTCGGTGGGCTCGCGATAGATCGGAAGAGCACACGTCT5229660.2961489542382687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGAGGCGCCTCGGTGGGCTCGCGATAGAGATCGGAAGAGCACACG4633150.26236935619696783No Hit
TCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT4537090.2569296013096275No Hit
AACCCGGCGGGCCAAGGTCGGCCGGCGCGGGAGATCGGAAGAGCACACGT4412390.24986799986832692No Hit
GGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACACG4297040.2433358769633227No Hit
AGCGCCGAGGCGCCTCGGTGGGCTCGCGATAGATCGGAAGAGCACACGTC4259710.2412219268285693No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA3969710.22479959789531204Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGTCTGA3705720.20985018198120664Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGACCTCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACA3471540.19658886822399912No Hit
CTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGAGCACACGTCTGAA3428540.1941538332442403Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCT3368090.19073062709246308Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTC3159140.17889805595244895No Hit
AGGGCGCCCGGGGTCCGCGGCGAAGATCGGAAGAGCACACGTCTGAACTC3150310.17839802435079152Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG2983950.16897727041514782TruSeq Adapter, Index 1 (100% over 49bp)
ACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAAC2896110.16400300360998465Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC2853510.16159062011840963Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGAG2840430.1608499164547993No Hit
ACGATTAACAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTCT2811650.15922014187997466Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG2806090.15890528619421979Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC2781480.15751165338371131Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACAC2748220.15562818214122806No Hit
CGGCGCGGGCGAGGCCGCGGGGGGCGCCGGGGGGAGATCGGAAGAGCACA2594150.14690339518003173No Hit
CGGTGGGGCGCGGGAGGTGTGGCGTACAGATCGGAAGAGCACACGTCTGA2588610.14658967206868606Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGAA2482670.14059042542320582Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACACGTC2466270.1396617144076699No Hit
TTCGGGGGACGGGCCCGCCGGCCCCCGGCGCCGCAGATCGGAAGAGCACA2454050.13896971144365472No Hit
CCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGAGCACACGTCTGA2438480.13808800226610018Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGACCGCGCGGCGCCGCCGGGCCGGGAGATCGGAAGAGCA2362470.133783653223973No Hit
CTAAATCATTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAA2357580.133506738780926Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGT2354780.13334817836363938No Hit
TCGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGACAGATCGGAAGAGCA2332620.1320932859182567No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC2296680.130058049704942No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACAGATCGGAAGAGCACACGTCTGAA2158270.12222006850614155Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGAAC1987070.11252523156347384Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT1969790.11154668727393355No Hit
CTCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1945300.1101598499098802No Hit
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC1928060.10918357076915829Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACA1905860.10792641317495723No Hit
GCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACG1882710.10661545829631963No Hit
GCGACCTCAGGTCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1801100.10199398841961921No Hit
AGCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGA1799450.10190055103086101No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC1791720.10146281102170902No Hit
TTCACTTACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC1778570.10071814334766649Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)