FastQCFastQC Report
Mon 26 Jun 2023
ERR2812341.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812341.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118024647
Total Bases6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC10614830.899374009565985No Hit
TGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCACAC10512950.8907419142715166No Hit
AGAAGTCAGCAACTTGATTCCAGCAGTAGTAGATCGGAAGAGCACACGTC9641380.8168954743834141No Hit
TTTGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCAC8517780.7216950201935363No Hit
GCGCCGAGGCGCCTCGGTGGGCTCGCGATAGATCGGAAGAGCACACGTCT8510420.7210714216328051Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGA8277200.7013111422396374No Hit
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCAC6390850.5414843562294238No Hit
AGCGCCGAGGCGCCTCGGTGGGCTCGCGATAGATCGGAAGAGCACACGTC6105810.5173334684915432No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT5912270.5009351987301432No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG5365320.45459318340515775Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACTCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTCT5036290.4267151080739941Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGGTCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC4765440.4037665115829578No Hit
GCAGTTTTACCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACA4486900.38016635626963574No Hit
GCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGAG4063850.34432214823739316No Hit
ACGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG3774170.3197781222764428Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACA3659020.31002168555522136No Hit
ACGATTAACAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA3627670.3073654607075419Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG3590480.3042144239584127No Hit
CTAAATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGA3401870.28823386355902425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAACGATGCCGACTCGCGATCCGGCGGCGAGATCGGAAGAGCACACGTC3349710.2838144476721036No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACAGATCGGAAGAGCACACGTCTGAA3250850.2754382311348917Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGA3092300.26200459637892415Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCACA2961880.2509543621003162No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2948680.24983595163813538Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCTCAGGTCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC2883450.2443091399375251No Hit
TGCAGTTTTACCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCAC2653820.2248530342988444No Hit
GCGACCTCAGGTCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA2648600.22441075379789105No Hit
GTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGT2544790.21561513333736132No Hit
GCGACCTCAGGTCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG2418250.2048936439521823No Hit
AGTTTTACCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACG2281050.19326895339072694No Hit
AGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC2148640.18205011026213871Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACG2056650.17425597553365274No Hit
GGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACA1960920.16614495784088218No Hit
AGGTCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGA1958340.165926359432365Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAAACGATGCCGACTCGCGATCCGGCGGCGAGATCGGAAGAGCACACGT1897170.16074354367694063No Hit
TGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTC1887360.1599123613561835No Hit
AGGTCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC1751620.1484113737700906Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1655300.14025036651878314No Hit
CGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1622930.1375077190444806No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG1501330.12720478630196624No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA1488070.1260812921558664No Hit
CGCGACCTCAGGTCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1457120.1234589585343136No Hit
TCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1449360.12280146874745576No Hit
GTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGAA1416760.12003933381813038Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1405610.11909461588984883No Hit
GGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACAC1391800.1179245213078248No Hit
AGGTCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAA1385280.1173720943219597Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT1369890.11606812939673525No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1353300.11466249079313069Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGCCTGGGAATACCGAGTGTCGTAGGCGTAGATCGGAAGAGCACACGT1349370.11432950949643594No Hit
CGGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACAC1294750.10970166256883615No Hit
AGGGCTTCTGCCGTGCGTGGCACGGGGGGAGATCGGAAGAGCACACGTCT1257530.10654808397774747Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACG1256040.10642183916042554No Hit
CGCGACCTCAGGTCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1255540.10637947512776716No Hit
TTTTGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCA1255270.10635659855013165No Hit
ACCTCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1253500.10620662987452104No Hit
GTGGAATTTTTTGAGGCTCCGCTTCGGCGAGATCGGAAGAGCACACGTCT1234400.1045883238269715Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGAATTTTTTGAGGCTTCGCTTCGGCGGAGATCGGAAGAGCACACGTC1228300.10407148262853944No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)