FastQCFastQC Report
Mon 26 Jun 2023
ERR2812342.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812342.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165667943
Total Bases16.7 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGA125133397.553265147983397No Hit
GCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGAG85604865.167255562532095No Hit
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCAC39578112.3890023189338447No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA34344782.073109581616523Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACA34234122.066429955009461No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA25987241.5686341925546816Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACAC19587461.1823325409430598No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA18151321.0956446776187714Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG17491661.055826473320792Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTG17210711.038867851458746Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA16144510.9745101983912482Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGA12581880.7594637666262326Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACA10375450.6262798832481429No Hit
CGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGACAGATCGGAAGAGCAC10345840.6244925730743214No Hit
GTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGTCTGAACT9722310.5868552372863107Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACGT9571750.5777671785301276No Hit
GGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACACG9093720.5489124712558301No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG8270120.49919856854865396Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCGGTCGGTGCGGAGCGCCGCTCGTGGTCGGGAAGATCGGAAGAGCAC8107080.4893571956766554No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC7875270.4753647481456325Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACG7475330.451223686648901No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT7112360.42931419749685673Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCACAC7078570.4272745753836033No Hit
CGGTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTG6734540.40650833698104166Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGACCTCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCACA6688180.4037099681982531No Hit
GGGCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGAGATCGGAAGAGCACAC6283270.3792689090127714No Hit
GCGCCGAGGCGCCTCGGTGGGCTCGCGATAGATCGGAAGAGCACACGTCT6213910.37508222094602817Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGCCAGATCGGAAG5776600.3486854424214104No Hit
TTTGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCAC5744950.3467749943632728No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA5703400.34426696539595475Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATTCAACCCGGCGGGCCAAGGTCGGCCGGCGCGGGAGATCGGAAGAGCA5621340.3393136836376365No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACAGATCGGAAGAGCACACGTCTGAA5562100.3357378560558333Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGCCGACCTCCGGGGACGCGTGCATTTATCAGAAGATCGGAAGAGCAC5499200.33194110462275733No Hit
CGGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGAAGATCGGAAGAGCACA5376470.32453291220015934No Hit
CGGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACAC5264220.31775731047738065No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG4823720.2911679781042492Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGTCTGA4652910.28085759476110594Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATTAACAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGA4331310.26144526946894003Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGAGCACACGTCTGAA4284930.2586456934519915Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC4077500.24612486436196046No Hit
CGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTCGGGAAGATCGGAAGAGCA3914930.23631186149272101No Hit
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC3629900.21910696386204298Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCTATTCTCAAACTTTCAATGGGTAAGACGAGATCGGAAGAGCACACG3602560.2174566747653769No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT3527750.2129410153900444Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCGAGGCGCCTCGGTGGGCTCGCGATAGAGATCGGAAGAGCACACG3526500.21286556325504688No Hit
CTAAATCATTCGTAGACGACCTGATTCAGATCGGAAGAGCACACGTCTGA3463670.20907303714153078Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGCCGAGGCGCCTCGGTGGGCTCGCGATAGATCGGAAGAGCACACGTC3384200.20427609220692744No Hit
GTGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGT3368080.20330306147399924No Hit
CGGTGGGGCGCGGGAGGTGTGGCGTACAGATCGGAAGAGCACACGTCTGA3272970.19756205942630675Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC3246310.19595281629107933Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCCAGATCGGAAGAGCACAC3202470.19330655901244576No Hit
TCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT3201070.19322205262124853No Hit
GCGACCTCAGGTCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC3161120.19081060238672728No Hit
GCGGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACG3008830.18161811787570756No Hit
CCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGAGCACACGTCTGA2968670.17919399168250674Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGCGCGGGAGGTGTGGCGTACGGAAGCAGATCGGAAGAGCACACGTC2821910.1703353074167161No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA2658930.16049755624719744Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC2647260.15979313511486046No Hit
GCGACCTCAGGTCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC2645270.15967301531594438No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCCCCAGATCGGAAGAGCAC2555080.15422899287160222No Hit
TTGGTGACTCTAGATAACCTCGAGCCGATCGCACAGATCGGAAGAGCACA2538970.15325656575575397No Hit
CGCGACCTCAGGTCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2497570.1507575910446356No Hit
CTCGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2480300.14971514434750965No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2451960.14800449354284553Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGACCGCGCGGCGCCGCCGGGCCGGAGATCGGAAGAGCAC2354680.14213250658879734No Hit
CGGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCCCCAGATCGGAAGAGCA2321130.14010737128546347No Hit
CGATTAACAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA2262800.13658647285793848Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGAGGCGCCTCGGTGGGCTCGCGATAGAGATCGGAAGAGCACACGT2248750.1357383908605662No Hit
GAGGCGCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGAGCACAC2238700.13513175569518598No Hit
GGGCGGTCGGTGCGGAGCGCCGCTCGTGGTAGATCGGAAGAGCACACGTC2211500.13348991723763962No Hit
ACGATTAACAGTCCTACGTGATCTGAGTTAGATCGGAAGAGCACACGTCT1996130.12048981618610426Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGGGGCGCGGGAGGTGTGGCGTACGGAAGCCAGATCGGAAGAGCACA1994120.12036848915302824No Hit
GCGACCTCAGGTCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA1957830.11817796276977978No Hit
GGTGGGGCGCGGGAGGTGTGGCGTACGGAAGAGATCGGAAGAGCACACGT1905990.115048811827162No Hit
GGGCCACGCCAGGGCGCCCGGGGTCCGCGGCGACAGATCGGAAGAGCACA1882670.11364117679664798No Hit
GTGGGGCGCGGGAGGTGTGGCGTACGAGATCGGAAGAGCACACGTCTGAA1844730.11135105359520278Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGTGGGGCGCGGGAGGTGTGGCGTACGGAAGAGATCGGAAGAGCACACG1806730.10905730869127772No Hit
CTAAATCATTCGTAGACGACCTGATTAGATCGGAAGAGCACACGTCTGAA1802080.10877662674908689Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGCGCGGGAGGTGTGGCGTACGGAAGCCAGATCGGAAGAGCACACG1771740.10694525252842671No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG1761980.10635612225836594Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGGCGCGGGAGGTGTGGCGTACGGAGATCGGAAGAGCACACGTCTG1761470.10632533778728694Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTGGGCTCGCGATAGCCGGCCGAGATCGGAAGAGCACACGTCTG1696220.1023867363404156Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCTGCCGGCGGGCAGGTGGCCCGGCGCCAGATCGGAAGAGCACACGT1669560.10077749320518815No Hit
CGCGACCTCAGGTCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1659990.10019983165964705No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)