FastQCFastQC Report
Mon 26 Jun 2023
ERR2812346.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812346.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137254501
Total Bases7.2 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC28978782.1113172820467287No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT21028181.532057589863665No Hit
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA16589811.208689688070776Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT11123630.8104382675217332No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC10522400.7666342395576521No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC8198920.5973516307490709No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT7653500.5576137718062886No Hit
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA5761070.4197363261697334Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT4340160.31621258089015236No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG3831900.27918210128496984No Hit
ATCCAGCTGGATAACGCAGAAGATATAGTCAGATCGGAAGAGCACACGTC2855740.20806166495042663No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT2736420.19936832526898335No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2304810.16792236197776858No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATTAAGATCGGAAGAGCACACG2197010.16006833903392356No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT1905570.13883479129037815Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCACGGTTTCCTTTGTGTCACGCTAGGGAGATCGGAAGAGCACACGTCTG1865520.13591685419482163Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC1710690.1246363498126739No Hit
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACAC1700370.12388446190190877No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCTG1638930.11940810596805128Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACG1636920.11926166268310574No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1588580.11573973810884353No Hit
ACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGTC1490870.10862084588395393No Hit
ATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGTCTGAAC1455640.10605408124284389Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT1414360.1030465295997834No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)