FastQCFastQC Report
Fri 23 Jun 2023
ERR2812347.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812347.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128933037
Total Bases13 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC38422782.9800570043192263No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT34592402.6829741084901304No Hit
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA21883181.6972515740864773Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC20919111.6224786514568799No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT16965151.3158109352531577No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG16009951.241725966634913No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA15346691.1902837594681028Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA11402810.8843978444407541Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCTG10067080.7807991058180069Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA9038430.7010173816040647Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC7975880.6186063855767239No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT6678700.5179975710957619No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT6168990.4784646467297594Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT6154850.4773679534129023Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG6022960.4671386124256113Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG6011980.46628700757277597No Hit
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA5742320.44537227491197623Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT5588110.43341180274842983Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT5433030.42138385369763687No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT5298510.4109505308557961No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC5119080.39703400455850585No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC5038990.39082225294980055No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT4823170.3740833313342336Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA4715230.3657115437372347No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT4318040.33490563012178176No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT4294510.3330806517805053Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG4016990.3115563003452715No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT3999400.3101920262686436No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC3943550.30586032034597926No Hit
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAAC3901940.3026330637042235Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC3697240.28675660529116365No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2886370.22386581958819446No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC2555570.19820909050641536No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC2528950.19614445287595297No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT2413500.18719019237869966No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC2316780.17968862394825927No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1917900.14875163454033893Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAGATCGGAAGAGCACACGTCTGAA1908480.14802102272670425Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAAC1888950.14650628294748072Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGTGTTCAAAGCAGGCCCGAGCCAGATCGGAAGAGCACACGTCTGAACT1832060.1420939149986826Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTTGGTCCGAGTGTTGTGGGTAGATCGGAAGAGCACACGTCTGAACTCC1815390.14080099579132693Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTG1749360.13567973272823783Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1717890.13323893084128624No Hit
ATCCAGCTGGATAACGCAGAAGATATAGTCAGATCGGAAGAGCACACGTC1649660.12794703656906803No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1640530.12723891705118215Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1632480.12661456194505058No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1611610.12499589224753932No Hit
AGTTGGTCCGAGTGTTGTGGGAGATCGGAAGAGCACACGTCTGAACTCCA1595630.12375648919213778Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1590870.12338730530329478Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT1567140.12154681503391562Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC1509600.1170840333187839Illumina DpnII expression Sequencing Primer (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG1501740.11647441454434987No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGAGATCGGAAGAGCACACGT1464940.1136202197734627No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA1431960.11106230282933614Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACACG1364470.1058278026910977No Hit
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTC1359280.105425268156834Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG1356810.10523369584476631Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1297080.1006010585169106Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)