FastQCFastQC Report
Sat 24 Jun 2023
ERR2812352.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812352.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58442622
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG16367482.8006067215806985No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT9172981.5695702359144668No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT7420131.269643583068535No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG4107540.7028329427108866No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3744160.6406557186979052No Hit
AAGGTGTCTGTTAATGAGAGAGTGATGCCAGATCGGAAGAGCACACGTCT3383120.5788788873983101Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA3041220.5203770631646198Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2875380.49200051291333236No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT2741400.46907546345199913No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC2617830.4479316482412442No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2536640.434039389950711No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTG2376010.40655431236469847Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA2314940.39610474697730025Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGATCAGCACCATGAAGCTTCTCACGGGAGATCGGAAGAGCACACGTCT2092970.35812390484465256Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGATCAGCACCATGAAGCTTCTCACGGGCAGATCGGAAGAGCACACGTC2081560.3561715625969006No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC1745480.2986655869067613Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1742700.29818990667461837No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT1703710.2915184058648156Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAA1578050.2700169749399676Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1559430.2668309440325932No Hit
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC1506660.2578015750217367Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT1490690.25506898030687264No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1435840.24568370666189482Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1251180.21408690390379817No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGATCGGAAGAGCACACGTCTGAAC1188520.20336527680089372Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT1070290.18313517829504639Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1057520.18095012917113815Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1022530.17496306035003015No Hit
AGCGATGCCAGAGAGAATATCCAGAGATTAGATCGGAAGAGCACACGTCT1011110.17300900702230643Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC1005460.172042246838275Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC1000120.17112853013336737No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC961430.16450836172271668No Hit
TGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTGA933930.15980289180043974Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG912380.1561155144613464No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG898440.1537302689807449Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT892160.15265571075849402No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC881720.15086934326800053No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC870790.14899913285889194Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA850600.14554446239595478Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCACCGTGAAGGTGCCTATGATGAAGCGTAGATCGGAAGAGCACACGTC846840.14490109632658163No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT825040.14117094198819485No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACT823640.14093139079215167Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCAGCACCATGAAGCTTCTCACGGGAGATCGGAAGAGCACACGTCTG798510.1366314468231764Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAATGCCCTGTGCAGAAGACTATCTATCAGATCGGAAGAGCACACGTCT787920.13481941313310686Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC782160.13383383106938632No Hit
AGATCAGCACCATGAAGCTTCTCACGGGCAGATCGGAAGAGCACACGTCT780120.13348477075515194Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT744550.1273984592956832No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA719000.12302664996789502Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT713300.12205133438400488Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA670720.11476555586434846Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA667830.11427105375251645Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGATGCCAGAGAGAATATCCAGAGATTCAGATCGGAAGAGCACACGTC651080.11140499479985685No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC642230.10989068902486955No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC610930.10453500871333253No Hit
AGACCAACCAAGATGAGTGCCCTGGGAGCAGATCGGAAGAGCACACGTCT599040.10250053462693717Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT596400.10204880951439857Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG595710.10193074499634874No Hit
AAACTGCGCACAGAAGGAGATGGAGTATAAGATCGGAAGAGCACACGTCT591460.10120353600836048Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACAC587700.10056016993898734No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)