FastQCFastQC Report
Sat 24 Jun 2023
ERR2812353.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812353.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114729720
Total Bases11.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG42984983.7466299054856926No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT18537001.6157103843711988No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT17553981.530029010791624No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT17278031.5059768297177052No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC12059781.0511469913811347No Hit
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC8409980.733025409632308Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC6566470.5723425455932429No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT5610800.48904503558450246No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA4059620.3538420559206455Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3727660.32490796630550484No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3515080.3063792014832774No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC3410540.2972673514761476Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC3362070.29304263969266203No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT3092860.26957792627751553Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG2948550.25699966843813443No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2663600.23216303500086985No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2578510.224746473712304No Hit
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA2577350.22464536651880612Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT2504620.21830611980923512No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG2340360.20398899256443753Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2332080.20326729639015942No Hit
AGAATGCCCTGTGCAGAAGACTATCTATCAGATCGGAAGAGCACACGTCT2302680.20070475200322985Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2174840.18956204198877152No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC2173900.18948011029748876No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT2111700.18405867285303232Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC2098400.18289942658275465No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC2097910.18285671750963917No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGAGATCGGAAGAGCACACGT2037780.1776157041087523No Hit
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA1987740.17325414896854974Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA1980380.1726126412580803Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGAAACCTATGGTGAAATGGCTGACTGAGATCGGAAGAGCACACGTCT1929570.16818397186012482Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1873960.16333692786838494No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTG1779130.15507141479993153Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1613540.14063836292810616Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC1579420.13766441685728859Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1546760.13481772639208045No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1467370.12789798493363358No Hit
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC1426350.12432262538425091Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1415990.12341963355266622Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGCGCAATGAAGGTGAAGGCCAGATCGGAAGAGCACACGTCTGAACT1404810.12244516939464334Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA1338730.11668554582021119Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA1269300.110633931643867Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT1243940.108423519206706No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG1232680.1074420821387867Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT1226960.1069435190811936No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAGATCGGAAGAGCACACG1218970.10624709970528998No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT1188170.10356252939517328Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTGA1185620.10334026789222532Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)