FastQCFastQC Report
Thu 6 Jul 2023
ERR2812354.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812354.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186609331
Total Bases18.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC50758732.720053157470459Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA49819962.6697464554974477No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACT33291391.7840152912825136Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG25801001.3826211080516653No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT21156861.1337514521179008No Hit
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT20805961.1149474620859126Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA18559440.9945611990860199Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT17708070.948938078557283No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA9035620.48419979598983715Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC7972800.4272455164634827Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT7435220.3984377394290107No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA7113130.38117761646120474Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA6574150.3522948163830028Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA6491420.347861490377456No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC6393490.34261362846855714No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCAGATCGGAAGAGCACACGTCTG6382520.34202576933304585Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG6347750.34016251845412815Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG6190100.3317143878512699Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAGATCGGAAGAGCAC5870740.314600559818737No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA5590390.29957719531184646No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC5565650.2982514309533643No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT5297260.2838689775914796No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC4588670.24589713576541358No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC4296620.23024679296449543No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC4277480.22922112078093246No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG4220990.2261939409664354Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTGGTCCGATGGTAGTGGGTTATCAGATCGGAAGAGCACACGTCTGAAC4100450.2197344569013004Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC4070870.21814932716306668Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG3920440.21008810111430065No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA3864620.20709682518501712Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCAGATCGGAAGAGCACACGTCTGA3794470.20333763481527084Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC3779460.20253328061071071No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGGAAGAGCACAC3657770.19601217047394057No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACGT3594180.1926045166519567No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC3576780.19167208739417216Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTCTG3563740.19097330132971754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTCTGA3502230.18767711031556078Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC3375650.18089395540462017Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGGAAGAGCACACGTCTGA3137230.1681175310574368Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC2886740.15469430089752587Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCAGATCGGAAGAGCACA2869400.15376508691304402No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC2791680.14960023622827306No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG2780420.14899683660513202No Hit
GGGCCGCCGGTGAAATACCACTACTCTAGATCGGAAGAGCACACGTCTGA2762870.14805636916409073Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACGT2750580.1473977740159199No Hit
GGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAACT2725270.14604146456106207Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGAA2712660.14536572128861017Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCT2627740.14081503780751456Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACG2552500.1367830850859221No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2551050.1367053826477734Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC2464970.13209253721615882No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2456330.13162953786056925No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACA2401270.12867898872645334No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2394390.1283103040544098No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT2353060.1260955166277296Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT2343620.12558964696143732Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGT2285640.1224826211932564No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCAGATCGGAAGAGCACACGTCTGAA2269940.12164129134571518Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2269520.12161878443259626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG2173390.11646738072277854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCAGATCGGAAGAGCACACGTCTGAAC2158180.11565230894054275Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCT2121850.11370546095575468Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG2099560.11251098692379964Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG2092580.11213694346291826No Hit
GGCGGAGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGT2076940.11129882888867974No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC2064690.11064237725604407No Hit
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT2015350.10799835084345275Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCAGTCGAGAGTGGACGGGAGCGGCAGATCGGAAGAGCACACGTCTGA2006400.10751873924246586Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT1980440.10612759766016203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGA1972020.10567638764001572Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT1942450.10409179378066578No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1926180.10321991883674884Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCTGA1908730.10228481018454538Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCCCGGCGGGGAAGGTGGCTCGGGGGGCAGATCGGAAGAGCACACGTC1896550.1016321097040962No Hit
GGAGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTG1884810.10100298789453353Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1877030.10058607412294941Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGA1869830.10020024132662478Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)