FastQCFastQC Report
Mon 10 Jul 2023
ERR2812358.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812358.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234825204
Total Bases11.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC61633982.6246748198289653No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCA36827381.5682890666199527No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC30534251.300296964715934No Hit
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTG30514961.299475502638124Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTAGTGGTATTTCACCGGCGGCCCGCAGATCGGAAGAGCACACGTCT28015581.193039738613407Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGT27487131.1705357658286117No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT24309921.0352347016379042No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT21731590.9254368623906316No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGT17870520.7610137112879927No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA13454220.572946164671489No Hit
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGT13216390.562818205834498No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG13122870.5588356691047525No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT13116320.5585567382281503No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACG12791090.5447068620453535No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT12333620.5252255630958592No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACAC12125550.5163649298905751No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG11642210.4957819604406689No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTG11568930.49266134141205725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC10230630.4356700143652382No Hit
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA9885630.4209782353686362Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG9843120.4191679526870548No Hit
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA9403850.4004616983107146Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGCTGTTCACCTTGGAGACCTGCTGCGGATAGATCGGAAGAGCACAC9128670.38874319470409147No Hit
AGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGATCGGAAGAGCACACG8730610.37179186268267866No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT8169790.34790941776420214No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAGATCGGAAGAGCACACG7718790.3287036429019774No Hit
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGT7593870.3233839413592078No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACAC7576510.3226446680740454No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCT7571120.32241513564276514Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGATCGGAAGAGCACA7536420.3209374407697736No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCAC7264460.30935606043378544No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTG6878260.29290978493092246Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC6645590.2830015640058807No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCT6606110.2813203134702696Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG6508300.27715508766256625Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC6310310.26872370991318295No Hit
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGT6217490.2647709825900971No Hit
GGAAGGACCCCACACCCCCGCCGCCGCAGATCGGAAGAGCACACGTCTGA5854340.2493062882636738Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCT5382550.2292151740236538Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACG5330130.22698287531350342No Hit
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTC5179420.22056491005965442No Hit
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAGATCGGAAGAGCACACGTC5148690.21925627710729043No Hit
AGGATTACCATGGCAACAACACATCATCAGTAGATCGGAAGAGCACACGT5141610.21895477625136014No Hit
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACA5130110.21846505028480673No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC5065260.21570342168211212No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA5049060.21501354684227164No Hit
CCGGCCGCGCCCCGTTTCCCAGGACGAAGGGCAGATCGGAAGAGCACACG4816360.2051040483712302No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCT4807050.2047075832626552Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACA4596310.19573324846339749No Hit
GGGAGAGTTCTTGGGTGGGTGTGGGTATAGATCGGAAGAGCACACGTCTG4567550.1945085076983474Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTAGGGACAGTGGGAATCTCGTTCATCCAGATCGGAAGAGCACACGTC4544030.19350691163457906No Hit
AGTACTTGGATGGGAGACCGCCTGGGAATAGATCGGAAGAGCACACGTCT4467790.19026024140066328Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACGAAGGGCACTCCGCACCGGACCCCGGTCAGATCGGAAGAGCACAC4424380.1884116323390908No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACG4302380.18321627860695908No Hit
AGGACCGACTGACCCATGTTCAACTGCTGTTCAGATCGGAAGAGCACACG4259980.18141068026071003No Hit
CCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGATCGGAAGAGCACAC4226020.17996449818904448No Hit
ATCTAAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGT4114660.17522224743814127No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTC4043600.1721961668135078No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATATTAGATCGGAAGAGCACACG4025650.17143176845701794No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCAGATCGGAAGAGCACACGT3985800.16973476152074374No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC3943870.16794917806182338No Hit
CTCGGGATCCCACCTCGGCCGGCGAGCGCGCAGATCGGAAGAGCACACGT3910180.16651449390415518No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCT3782910.16109471792474203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTG3739290.1592371660411716Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGAGGAAGGACGCGCGAGGGCCGGGACCCCAGATCGGAAGAGCACACG3720370.15843146036402464No Hit
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGT3694350.15732340213361423No Hit
GTGGAGGGGGGGGCGGCCCGCCGGCGGGGACAGATCGGAAGAGCACACGT3638680.1549527026068292No Hit
ACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCAC3611010.15377437934643506No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT3507560.1493689748907873No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT3433350.14620875193618485No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGATCGGAAGAGCAC3428410.14599838269490018No Hit
CTCACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGT3371500.1435748779334607No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT3371050.1435557147434651No Hit
AGCTAGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGT3325470.14161469652124736No Hit
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCT3300060.1405326150594976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA3254680.13860011381061121No Hit
CCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGATCGGAAGAGCAC3237390.13786382146611487No Hit
AACACCTTTTCTGGGGTCTGATGAGCGTCGGCAGATCGGAAGAGCACACG3183220.13555699923931502No Hit
ATGATTAAGAGGGACGGCCGGGGGCATTCGTAGATCGGAAGAGCACACGT3177640.13531937568337No Hit
ACCGGATAAAACTGCGTGGCGGGGGTGCGTAGATCGGAAGAGCACACGTC3163340.1347104120901775No Hit
GAACGGGGAGGAGGCGGGAACCGAAGAAGCAGATCGGAAGAGCACACGTC3142520.13382379516638257No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACA3109990.1324385094540363No Hit
AGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCGGAAGAGCACA3086570.13144117187693363No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTC3076770.1310238401836968No Hit
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCAC3002190.12784786082843136No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC2983250.12704130345395123No Hit
ACTGGGAATTCCTCGTTCATGGGGAATAATTGCAGATCGGAAGAGCACAC2950710.12565559189293837No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTCT2907860.12383083035669375Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG2883840.12280794185959697No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG2881640.12271425515295198No Hit
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTC2824830.12029500887817818No Hit
CCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCAGATCGGAAGAGCACA2811740.11973757297364042No Hit
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTC2807400.11955275465234984No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT2656340.11311988469517097Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC2630180.11200586458342862No Hit
GGAAGGACCCCACACCCCCGCCGCAGATCGGAAGAGCACACGTCTGAACT2481940.10569308395022196Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGATCCAACTACGAGCTTTTTAACTGCAGCAGATCGGAAGAGCACACGT2441680.10397861721861851No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAGATCGGAAGAGCACACGT2414230.10280966262888884No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)