FastQCFastQC Report
Thu 29 Jun 2023
ERR2812359.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812359.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148037048
Total Bases11.7 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2341950715.82003107762592No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT36827332.4877103736896995No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC27780391.8765836238507No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT27693621.8707222532564955No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG18789981.2692755127081432No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT15161391.0241618706149829No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG15086981.0191354261535936No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA12985990.8772121692132093No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC11876230.8022471509969585No Hit
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA8939220.6038501929598056Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG8881690.5999640036053678Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC7830150.5289317846975711No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA6974680.47114422330280453No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT6878540.4646499030431896No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG5919280.3998512588551482No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT5324620.35968158457199173No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC5182720.3500961462025371No Hit
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA5112450.3453493614652462Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG5005420.3381194145400684No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT4836640.3267182144837149No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA4467990.3018156644139513Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT4325860.29221468939315787No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG4238570.28631819245679635No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG4222030.28520090457356323No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT4048740.2734950510496535No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT3788470.2559136412933606Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC3763250.25421001369873303No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT3723970.2515566238526994No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC3477530.23490943969647382No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG3453830.2333084891019983No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT3406680.23012347557754598No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT3161540.21356410727671357No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC2912630.19675007299524103No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT2790350.18848997853564334Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACAC2775430.18748212271836168No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2718610.18364389433110015Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2566300.17335525361192017Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC2537750.17142668232617014No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG2484000.16779583445895246No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACG2465690.16655898191106866No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG2247280.15180524269843587No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2093880.14144297176204162No Hit
ACCATTTCTTCCAGAGAGATTCAGACAGCAGATCGGAAGAGCACACGTCT2092970.1413815006632664Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCAGCTGGATAACGCAGAAGATATAGTCAGATCGGAAGAGCACACGTC2008720.13569035772720894No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCAC1976430.13350914698055855No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1971200.133155857039246Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGTCTG1953920.13198858166909677Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1912990.12922373323737177No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT1889630.12764574986661448No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCGAGATCGGAAGAGCA1858720.12555775902799682No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCAGATCGGAAGAGCA1804050.12186476455542399No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA1701040.11490637127538506Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCGAGATCGGAAGAGC1692610.11433691922848935No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1654190.11174162294833115No Hit
GCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACA1637580.11061960651903839No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC1619570.10940301916855301No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1608070.10862618660161341Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGGGCGTGGAATGCGAGTGCCTAGTGGGAGATCGGAAGAGCACACGTC1521890.10280467089562607No Hit
AGATCGGAAGAGCACACGTCTACAGAGTTCTACAGTCCGACGATCAGATC1514320.10229331241460585Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACACG1504580.101635369005737No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1494340.10094365026787078No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)