FastQCFastQC Report
Wed 19 Jul 2023
ERR2812360.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812360.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219524298
Total Bases22.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC73932783.367863178407704No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT45939262.0926731308804825No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT39411831.7953288250578985No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA29893171.3617248875110854Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT28542521.3001986686685592No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG22239591.0130810212179793No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA18931820.8624020289544441Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT16420990.7480260795549839No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC16356190.7450742423055147No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC16249820.7402287650180756Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG16210050.7384171204592578No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA14700050.6696320240595872Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT13163990.5996598153339727No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG12443550.5668415803338545No Hit
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAAC11492800.5235320237762473Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG11206670.5104979313041693Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG10616720.48362391301212593Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT9620240.4382312157536201Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC9330490.4250322212623589Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG9142810.41648282596945146Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC9083970.413802484862063No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGGAAGAGCACACGTCTGA8653080.3941741337444113Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT8632500.393236652099441Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG8519010.38806683713891205No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA8384020.3819176317329574Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT8352990.3805041207784662Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG8176270.3724539868475061No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC8155310.37149919504582585Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACT8066330.36744588519308236Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA8025530.36558732099897207Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGTTGGCCTCGGATAGCCGGTCCCCAGATCGGAAGAGCACACGTCTGA7420220.3380136079514989Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG7391540.33670714665034485No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC7372260.33582888396253974No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT7160820.32619714834482694No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA7160170.32616753886624433Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGAA7131940.3248815764348783Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG6971220.31756029120749085Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT6426620.29275210345963615Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC6212980.2830201511451821No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC6176370.2813524542053199No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT6166210.28088963527855126Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA6068690.2764473024302759Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT6000620.2733465067270139Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT5948440.27096954889248753No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT5661450.2578962808025925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC5654500.25757968714697815Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT5270490.24008686273079438No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC5247660.23904688673688415No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTG5241600.238770835290406Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC5141050.23419047671889148No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC4774740.217503941181035No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG4699720.2140865518221587No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG4603900.20972165914863783No Hit
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT4547990.20717478846009107Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC4480610.20410542435717072No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA4469090.20358065329059838No Hit
CCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGA4367490.19895246402291197Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC4321070.19683789172167174No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA4273860.19468733251569265Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACG4201390.19138610341894818No Hit
CGTGGCGCAATGAAGGTGAAGGCCGAGATCGGAAGAGCACACGTCTGAAC4072910.18553344832925966Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG3904280.1778518385240435Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC3773060.17187436809386814No Hit
AGCGAGCGCACGGGGTCGGCGGCGACAGATCGGAAGAGCACACGTCTGAA3738960.17032100929437888Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTC3730510.16993608607280458No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACGT3633980.1655388507380627No Hit
GCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTG3633820.165531562251027Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC3559650.16215289297952795Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC3484040.15870862732470736Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC3432590.1563649232122815No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT3407900.15524021855658093No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC3397160.15475097886430775No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGATCGGAAGAGCACACGTCTGAAC3355500.15285323905238044Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG3343490.1523061469942612Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT3323600.15140009694963244No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC3299800.1503159345030681Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT3133720.1427504849599838No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG3051910.1390237904325288Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA2882270.1312961720528996Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA2880920.13123467544353565Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTGAACTC2833600.12907910540271947Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT2824810.12867869414619423Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT2804110.1277357461359471No Hit
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTC2782010.12672902386413734Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA2757600.12561707406074932No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG2749370.12524217250884911No Hit
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTC2719300.12387239247657222Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA2706110.1232715478265645Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGATAGCTGGCGCTCTCGCAGACCCGAGATCGGAAGAGCACACGTCTGA2700270.12300551804976048Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACAC2659610.12115333128180646No Hit
GGGCCGCCGGTGAAATACCACTACAGATCGGAAGAGCACACGTCTGAACT2622630.1194687797156741Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAACT2556140.11643995782188994Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA2553240.11630785399436741Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT2522980.1149294188837356Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT2512740.1144629557134491No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT2483780.11314373955998255Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC2436410.11098589186696772No Hit
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA2434950.11091938442276672Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT2413770.10995457095141241Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC2405340.10957055879071756No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC2392020.10896379224499331No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT2390500.10889455161815391Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA2371080.10800991150419258Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT2356550.10734802577526065No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCAGATCGGAAGAGCACAC2291750.10439618852579133No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG2270320.10341998679344369No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT2268840.10335256828836323Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT2230650.10161289753902322Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGCACACGTCT2221420.10119244294314973Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC2213520.10083257389576074No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAGATCGGAAGAGCACACG2202320.10032237980325986No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT2199030.10017251028858773Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)