FastQCFastQC Report
Fri 23 Jun 2023
ERR2812364.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812364.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96579453
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC17017531.7620238540800186No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC9483890.9819780196932778No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT9235850.9562955383480999No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT6705400.6942884631993101No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA6237900.6458827220734Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG5506130.5701140179371279No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT4885640.505867433314206No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC4349220.45032559875856826Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACCTGCATTAAAAATTTTGGTTGGGAGATCGGAAGAGCACACGTCTGA4067290.42113408946310765Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT3746350.38790341875305506No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA3579000.370575716555363Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTCAACCTTAAGCTTACCTATAGAACCAAGATCGGAAGAGCACACGTC3134680.32457007185576003No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3116450.3226825068060802No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3110300.32204572539875537No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC2991700.30976568069815014No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2740580.2837642909408485Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT2649970.274382378206263No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2529710.26193045429652617No Hit
AGTTCAACCTTAAGCTTACCTATAGAACAGATCGGAAGAGCACACGTCTG2332940.24155655551289984Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG2323100.24053770526118015No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC2319300.240144246830638Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG2205830.22839537101126467No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2076230.21497636769593217No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG2018670.20901650789014098No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC1930310.19986756396311336No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1751350.18133774271842273No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT1669530.1728659614586966No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC1668820.17279244685720058Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1623470.16809683111375667Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1546040.160079597882999Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1513560.15671656371878603No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG1475040.15272813773339552Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT1452350.15037877673628985No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC1410710.1460673006710858No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1402560.14522343587926514No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1375020.14237189767475697No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCAGATCGGAAGAGCA1306220.13524822924809896No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1298960.13449651656237896No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGC1260430.130507055160066No Hit
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT1257200.13017261549410516Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG1238080.1281928983383246No Hit
ATTCTAGAGTCCATATCGACAATAGAGCAGATCGGAAGAGCACACGTCTG1158510.11995408588615634Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1144220.11847447510393333No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC1121710.1161437516114323No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT1104250.11433591366478332Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT1076040.11141500252646906No Hit
AGTTCAACCTTAAGCTTACCTATAGAACCAGATCGGAAGAGCACACGTCT1074310.11123587539888012Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGAAAAAACCTTATAGAGAGAGTAAAAGATCGGAAGAGCACACGTCTG1069680.1107564773637722Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1013150.10490326550099636No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC1008660.10443836330280314No Hit
GTTCAACCTTAAGCTTACCTATAGAACCAAGATCGGAAGAGCACACGTCT1005110.10407079029532296Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA998660.10340294638032377No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT974250.10087549367255165No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)