FastQCFastQC Report
Sun 2 Jul 2023
ERR2812365.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812365.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72102095
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC12660521.755915691492737No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT5373830.7453084407602858No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC4535790.6290788083203408No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT4259640.5907789503203755No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG3939990.54644598052248No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA3937540.5461061845706425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT3582190.49682190233168677No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC3380390.46883381127829926No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA3188480.44221738633253305No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA2897070.4018010849754088Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG2869210.39793711958022854No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2674760.3709684163823534No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT2582480.3581698978372265No Hit
AGTTCAACCTTAAGCTTACCTATAGAACCAAGATCGGAAGAGCACACGTC2298440.3187757581801195No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGC2121830.29428132428052195No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1893690.2626400800143186Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC1792640.24862523065383885No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG1739450.24124819119333493No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT1591570.22073838492487632No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1459520.2024240765819634Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC1455070.20180689617964637Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT1412640.1959221850627225Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1337590.18551333355847704No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA1320100.1830876065390333No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1244140.17255254510981963Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1170690.1623656011659578No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGAGATCGGAAGAGCACACGTC1134690.15737268105732574No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1128130.15646286005975277No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1082720.15016484611161438No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC1074570.14903450447591018No Hit
TATTCTAGAGTCCATATCGACAATAGAGCAGATCGGAAGAGCACACGTCT1038770.14406932281232607Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTATTCTAGAGTCCATATCGACAATAGAGCAGATCGGAAGAGCACACGTC999790.13866309987247943No Hit
CTAGGAAAAAACCTTATAGAGAGAGTAAAAAGATCGGAAGAGCACACGTC991180.13746895981316493No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC922690.12796992930649242No Hit
CGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTG910340.12625708032478114Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA894790.12410041622230256No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCAGATCGGAAGAG890050.12344301507466601No Hit
ACTAGGAAAAAACCTTATAGAGAGAGTAAAAAGATCGGAAGAGCACACGT887650.12311015373409054No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC887170.12304358146597544No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT871630.12088830428574925No Hit
GCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTC852480.11823234817240748No Hit
GGTCGATGATGAGAACCTTATATTGTCTGAAGAGATCGGAAGAGCACACG826600.11464299338320198No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG824900.11440721660029435No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC814150.11291627517896671No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC809470.11226719556484453No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT793320.1100273161272221No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG777040.10776940670031848Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT766910.10636445445863951Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCTAGAGTCCATATCGACAATAGAGCAGATCGGAAGAGCACACGTCTG757700.10508709906418114Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG753200.10446298405060214Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTATTCTAGAGTCCATATCGACAATAGAGCAGATCGGAAGAGCACACGT750600.1041023842649787No Hit
CCCCACAACCGCGCTTGACTAGCTTGACGTTTAGATCGGAAGAGCACACG732900.1016475318782346No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG729670.10119955599071012No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT729540.10118152600142895No Hit
TAGGAAAAAACCTTATAGAGAGAGTAAAAAGATCGGAAGAGCACACGTCT725130.10056989328812152Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC722700.10023287118078884Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA721970.10013162585636382No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)