FastQCFastQC Report
Sat 24 Jun 2023
ERR2812366.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812366.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65137295
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length51-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA12998751.9955925403411363Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC9370121.4385184401654996No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG6319660.9702060854691616Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA5731200.8798645998425326Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT5277500.8102117227926029No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT3214120.49343774561102055No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG3032400.46553974953979893No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA2143520.3290772206613738No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT1894120.29078886373774043No Hit
TATTCTAGAGTCCATATCGACAATAGAGCAGATCGGAAGAGCACACGTCT1721530.26429252243280904Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1706670.26201118729293255No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG1538550.23620108879252663Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1482700.22762689178296397No Hit
ATTCTAGAGTCCATATCGACAATAGAGCAGATCGGAAGAGCACACGTCTG1440510.22114980365702938Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1438520.22084429511541737No Hit
GACTATATCTTCTGCGTTATCCAGCTGGATGAGATCGGAAGAGCACACGT1398400.2146849972815113No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT1358280.20852569944760524No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG1309010.20096167640980483No Hit
AGTTCAACCTTAAGCTTACCTATAGAACCAAGATCGGAAGAGCACACGTC1262650.19384440204340692No Hit
CTATTCTAGAGTCCATATCGACAATAGAGCAGATCGGAAGAGCACACGTC1238080.19007236944672018No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCAGATCGGAAGAGCA1112370.1707731338858944No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1099690.16882647644486926Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1059030.1625842767956514No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1043240.16016016630718238No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1032970.15858349659745619Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG975170.14970993192148982No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT964090.14800890948879594No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT962650.1477878379812978No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGC961970.14768344310275705No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA958760.14719063786729247Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTATTCTAGAGTCCATATCGACAATAGAGCAGATCGGAAGAGCACACGT926730.1422733320442613No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC896430.13762161907398826No Hit
GGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCAC853820.13108005175836668No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTC844280.12961545302119162No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT800340.1228697016048947Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG787810.12094607244590061No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT746890.11466395710782894No Hit
CTAGGAAAAAACCTTATAGAGAGAGTAAAAGATCGGAAGAGCACACGTCT739490.11352789519429692Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA739390.1135125430062762Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACG733720.11264207394550234No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTG719820.11050811981062462Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC699470.10738394954841156No Hit
ACTAGGAAAAAACCTTATAGAGAGAGTAAAAAGATCGGAAGAGCACACGT696170.10687732734372835No Hit
CTAGGAAAAAACCTTATAGAGAGAGTAAAAAGATCGGAAGAGCACACGTC677560.10402028515307553No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC675040.10363341001495381No Hit
TAGGAAAAAACCTTATAGAGAGAGTAAAAAGATCGGAAGAGCACACGTCT673580.10340926806985153Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)