FastQCFastQC Report
Sat 24 Jun 2023
ERR2812370.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR2812370.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72340254
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC19802462.737405373224153No Hit
ACACCTGCATTAAAAATTTTGGTTGGGAGATCGGAAGAGCACACGTCTGA13051541.8041877486357734Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT10512971.4532669459523877No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA7504351.0373684891955175Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG5645050.7803469973992626Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG4048710.5596759447374902Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA3873660.5354777991241225Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT3749360.518295111322114No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC3555240.49146081239913814No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG3124980.431983553721003No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2931300.4052100784716625No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT2793450.3861542979929266No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2694250.37244132430057547No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2665860.3685168149948713No Hit
GGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTC2585080.35735014145789423No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2318950.3205614954019929No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC2217940.3065983152340051No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2031920.28088372484840873No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1914250.2646175392195886No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG1789260.24733946883846994No Hit
GTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGC1697800.23469643885961475No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1678730.23206028555000652No Hit
CGTGAAACCTATGGTGAAATGGCTGACTGAGATCGGAAGAGCACACGTCT1555940.215086333537065Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1540130.21290082835484656No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1533010.21191659072692776No Hit
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA1485630.20536698696136732Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG1473490.20368880651151708No Hit
ACATAAAGCACCTGGCTTACACCCAGAAAAAGATCGGAAGAGCACACGTC1427850.1973797327280604No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1333300.18430955467753818No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1283370.17740745007613604Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGA1128250.15596434040720952Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG1053580.14564228652003353No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCT998300.1380006213414733Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG993960.13740067874243295Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT987530.1365118236936243No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT939140.12982260194994616Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGAAAAAACCTTATAGAGAGAGTAAAAGATCGGAAGAGCACACGTCTG859160.11876651691048803Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT848510.11729430753726687No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA832800.11512262591723828No Hit
AGCTGCGAGAATTAATGTGAATTGCAGATCGGAAGAGCACACGTCTGAAC782060.10810855046209818Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACCTGCATTAAAAATTTTGGTTGGGAGATCGGAAGAGCACACGTCTGAA744760.10295236176527663Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTATATCTTCTGCGTTATCCAGCTGGATAAGATCGGAAGAGCACACGT742700.10266759638416531No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)